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Studies on an ultraviolet-absorbing photopigment, Opsin 5 in vertebrate eye and brain

Research Project

Project/Area Number 24500382
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neuroscience in general
Research InstitutionOkayama University

Principal Investigator

OHUCHI Hideyo  岡山大学, 医歯(薬)学総合研究科, 教授 (00253229)

Co-Investigator(Renkei-kenkyūsha) BANDO Tetsuya  岡山大学, 大学院医歯薬学総合研究科, 助教 (60423422)
FUJITA Hirofumi  岡山大学, 大学院医歯薬学総合研究科, 助教 (20423288)
YASUE Akihiro  徳島大学, 大学院HBS研究部, 講師 (80380046)
Research Collaborator INOUE Junji  
KATO Mutsuko  
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsオプシン5 / Opsin 5 / Opn5 / 光 / 紫外光 / 眼 / 脳 / 視床下部 / 神経内分泌 / opsin 5 / 網膜 / 光受容 / 視物質 / GPCR / 発生分化
Outline of Final Research Achievements

This research project was aimed for clarifying the functions for an ultraviolet-absorbing photopigment, Opsin 5 in vertebrates such as mouse and chicken by use of a genome-editing technique, TALEN (Transcription activator-like effector nuclease). We succeeded in production of Opn5-knockout mice by this method, finding that Opn5-deficient mice were born alive and seemed healthy at least after 3 weeks postnatal.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (24 results)

All 2016 2015 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (15 results) (of which Invited: 1 results) Book (3 results)

  • [Journal Article] Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation.2014

    • Author(s)
      Yamashita T*, Ono K*, Ohuchi H*, Yumoto A, Gotoh H, Tomonari S, Sakai K, Fujita H, Imamoto Y, Noji S, Nakamura K, Shichida Y. (*equally contributed)
    • Journal Title

      J Biol Chem

      Volume: 289 Pages: 3991-4000

    • NAID

      120005690203

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The expression of LIM-homeobox genes, Lhx1 and Lhx5, in the forebrain is essential for neural retina differentiation.2013

    • Author(s)
      Inoue J, Ueda Y, Bando T, Mito T, Noji S, Ohuchi H.
    • Journal Title

      Dev Growth Differ

      Volume: 55 Issue: 7 Pages: 668-675

    • DOI

      10.1111/dgd.12074

    • NAID

      40019813303

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular aspects of eye evolution and development: from the origin of retinal cells to the future of regenerative medicine.2013

    • Author(s)
      Ohuchi H
    • Journal Title

      Acta Med Okayama

      Volume: 67 Pages: 203-312

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken2012

    • Author(s)
      Kawaue, T., Okamoto, M., Matsuyo, A., Inoue, J., Ueda, Y., Tomonari, S., Noji, S., Ohuchi, H
    • Journal Title

      Biology Open

      Volume: 1 Issue: 11 Pages: 1083-1093

    • DOI

      10.1242/bio.20121396

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases.2012

    • Author(s)
      Watanabe T, Ochiai H, Sakuma T, Horch HW, Hamaguchi N, Nakamura T, Bando T, Ohuchi H, Yamamoto T, Noji S, Mito T.
    • Journal Title

      Nature Communications

      Volume: 3 Issue: 1 Pages: 1017-1017

    • DOI

      10.1038/ncomms2020

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Wakayama symposium: epithelial-mesenchymal interactions in eyelid development.2012

    • Author(s)
      Ohuchi H.
    • Journal Title

      Ocul Surf

      Volume: 10 Issue: 4 Pages: 212-216

    • DOI

      10.1016/j.jtos.2012.07.005

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Immunohistochemical analysis of opsin 5, an ultraviolet-absorbing photopigment, in chicken and mouse neural tissues2015

    • Author(s)
      Mutsuko Kato, Katsuhiko Ono, Takahiro Yamashita, Satomi Katayama, Keita Sato, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Yoshinori Shichida, Hideyo Ohuchi
    • Organizer
      第120回日本解剖学会(第92回日本生理学会合同大会)
    • Place of Presentation
      神戸市
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Comprehensive expression pattern analysis of a tumor suppressor gene, REIC/Dkk3 in the mouse2015

    • Author(s)
      Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi
    • Organizer
      第120回日本解剖学会(第92回日本生理学会合同大会)
    • Place of Presentation
      神戸市
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] CRISPR/CasシステムによるPax6遺伝子破壊マウスの解析2014

    • Author(s)
      河野仁美、泰江章博、石丸善康、井上順治、渡辺崇人、板東哲哉、親泊政一、野地澄晴、三戸太郎、田中栄二、大内淑代
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Molecular properties of Opn5L1, a photoreceptor protein found in non-mammalian vertebrates2014

    • Author(s)
      Keita Sato, Takahiro Yamashia, Hideyo Ohuchi, Sayuri Tomonari, Atsuko Takeuchi, Sari-Fujita-Yanagibayashi, Kazumi Sakai, Yasushi Iwamoto, Sumihare Noji, Akimori Wada, Yoshinori Shichida
    • Organizer
      16th International Conference on Retinal Proteins-ICRP2014-
    • Place of Presentation
      長浜市
    • Year and Date
      2014-10-05 – 2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Studies of Opsin 3-related proteins implicate the presence of novel photoreceptors in the chicken retina2014

    • Author(s)
      Hideyo Ohuchi, Takashi Sugiyama, Hirofumi Fujita, Mutsuko Katoh, Hitomi Kohno, Tetsuya Bando
    • Organizer
      第55回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      松本市
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 非哺乳類脊椎動物が持つ光受容タンパク質Opn5L1の光不活性化2014

    • Author(s)
      佐藤恵太,山下高廣,大内淑代,友成さゆり,湯本茜,柳林彩理,酒井佳寿美,野地澄晴,七田芳則
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      仙台市
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] コオロギにおけるエピジェネティック因子E(z)の概日リズムおよび再生への関与2014

    • Author(s)
      濱田良真,板東哲哉,大内淑代,富岡憲治
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      仙台市
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Localization of the two opsin 3-related proteins implicates the presence of novel photoreceptors in the chicken retina2014

    • Author(s)
      Ohuchi H, Fujita H, Inoue J, Bando, T.
    • Organizer
      第119回日本解剖学会総会・全国学術総会
    • Place of Presentation
      栃木県下野市
    • Related Report
      2013 Research-status Report
  • [Presentation] 可視光感受性タンパク質Opn5n の分子特性解析2013

    • Author(s)
      山下高廣,大内淑代,湯本茜,佐藤恵太, 友成さゆり,木下政人,野地澄晴,七田芳則
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      岡山市
    • Related Report
      2013 Research-status Report
  • [Presentation] 細胞の多様性を決めるしくみー眼の再生医療に向けてー2013

    • Author(s)
      大内淑代
    • Organizer
      日本実験動物技術者協会第47回総会
    • Place of Presentation
      倉敷市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 新規オプシン5様視物質(cOpn5n)のニワトリ網膜における発現様式2013

    • Author(s)
      大内淑代、山下高廣、湯本茜、佐藤恵太、井上順治、友成さゆり、野地澄晴、七田芳則
    • Organizer
      第54回日本組織化学会総会・学術集会
    • Place of Presentation
      東京都
    • Related Report
      2013 Research-status Report
  • [Presentation] A non-mammalian type opsin 5 functions dually in the photoreceptive and non-photoreceptive organs of birds2012

    • Author(s)
      Hideyo Ohuchi, Takahiro Yamashita, Sayuri Tomonari, Sari Fujita-Yanagibayashi, Kazumi Sakai, Sumihare Noji, Yoshinori Shichida
    • Organizer
      第14回国際組織細胞化学会議 (ICHC2012)
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] 非哺乳類タイプのオプシン5はニワトリ光受容器官と非光受容器官の両方に存在する2012

    • Author(s)
      大内淑代、山下高廣、友成さゆり、藤田(柳林)彩理、酒井佳寿美、野地澄晴、七田芳則
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] 脊椎動物非視覚オプシンOpn5とその類似光受容タンパク質の分子的性質の解析2012

    • Author(s)
      Takahiro Yamashita, Hideyo Ohuchi, Sayuri Tomonari, Sari Fujita-Yanagibayashi, Kazumi Sakai, Sumihare Noji, Yoshinori Shichida
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] Molecular properties of vertebrate non-visual opsins2012

    • Author(s)
      Takahiro Yamashita, Hideyo Ohuchi, Sayuri Tomonari, Sari Fujita-Yanagibayashi, Kazumi Sakai, Sumihare Noji, Yoshinori Shichida
    • Organizer
      15th international Conference on Retinal Proteins
    • Place of Presentation
      Ascona, Switzerland
    • Related Report
      2012 Research-status Report
  • [Book] 「脳内光受容体」光と生命の事典(真嶋哲朗、飯野盛利、七田芳則、藤堂剛編集、分担執筆)2016

    • Author(s)
      大内淑代
    • Total Pages
      450
    • Publisher
      朝倉書店
    • Related Report
      2014 Annual Research Report
  • [Book] 実験医学別冊 今すぐ始めるゲノム編集(山本卓編、分担執筆)2014

    • Author(s)
      渡辺崇人、三戸太郎、大内淑代、野地澄晴
    • Total Pages
      10
    • Publisher
      羊土社
    • Related Report
      2013 Research-status Report
  • [Book] ルイス・ウオルパート著「発生生物学 (サイエンス・パレット) 生物はどのように形づくられるか」2013

    • Author(s)
      大内淑代、野地澄晴(訳)
    • Total Pages
      181
    • Publisher
      丸善出版
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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