Analysis of telencephalin-dependent neuronal clearance for apoptotic cells
Project/Area Number |
24500392
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neuroscience in general
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Yutaka Furutani 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 研究員 (80392108)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 細胞接着分子 / 細胞外マトリックス / 細胞死 / ファゴサイトーシス / テレンセファリン / アポトーシス / ビトロネクチン |
Outline of Final Research Achievements |
I hypothesize that dead cells could be phagocytosed by neurons as a clearance mechanism in the brain. After induction of apoptosis, vitronectin bound to plasma membrane of cultured hippocampal neurons. Fragments of apoptotic cells interacted with neurons in a telencephalin-dependent manner and induced telencephalin accumulation. Vitronectin-coated magnetic beads used as an imitation of dead cells enhanced formation of phagocytic membrane protrusions on cultured hippocampal neurons and were collected with the membrane using a magnet apparatus. The beads-binding proteins were comprehensively analyzed by a mass spectroscopy. In this fraction, several proteins worked in phagocytosis were present. Thus, vitronectin bound to a surface of dead cells appears to be recognized by telencephalin and regulate a clearance of dead cells.
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Report
(5 results)
Research Products
(11 results)
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[Journal Article] Vitronectin induces phosphorylation of ezrin/radixin/moesin actin-binding proteins through binding to its novel neuronal receptor telencephalin.2012
Author(s)
Furutani, Y., Kawasaki, M., Matsuno, H., Mitsui, S., Mori, K. and Yoshihara, Y.
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Journal Title
J. Biol. Chem.
Volume: 287
Issue: 46
Pages: 39041-3904
DOI
Related Report
Peer Reviewed
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