| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
I hypothesize that dead cells could be phagocytosed by neurons as a clearance mechanism in the brain. After induction of apoptosis, vitronectin bound to plasma membrane of cultured hippocampal neurons. Fragments of apoptotic cells interacted with neurons in a telencephalin-dependent manner and induced telencephalin accumulation. Vitronectin-coated magnetic beads used as an imitation of dead cells enhanced formation of phagocytic membrane protrusions on cultured hippocampal neurons and were collected with the membrane using a magnet apparatus. The beads-binding proteins were comprehensively analyzed by a mass spectroscopy. In this fraction, several proteins worked in phagocytosis were present. Thus, vitronectin bound to a surface of dead cells appears to be recognized by telencephalin and regulate a clearance of dead cells.
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