Structural analysis of excitatory synapse by using high-resolution imaging method
| Project/Area Number |
24500405
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IWASAKI Hirohide 東京大学, 医学(系)研究科(研究院), 講師 (30342752)
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| Co-Investigator(Renkei-kenkyūsha) |
OKABE Shigeo 東京大学, 大学院医学系研究科, 教授 (60204012)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | シナプス / 光学顕微鏡 / 電子顕微鏡 / コネクトーム |
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| Outline of Final Research Achievements |
Neurons are connected via synapses to form neural circuits. In order to understand how neurons are involved in various brain functions at neural circuit level, it will be useful to analyze the wiring diagram of the brain at synapse level with high-resolution imaging technique which can cover large area. From this point of view, I established the hybrid method of light and electron microscopy, Array Tomography, for the three-dimensional reconstruction of neural tissue and synapse localization. I also established the method to observe synapses by using scanning electron microscopy. By using these technologies, I analyzed the morphology of excitatory synapses at developmental stage of mice.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Doublecortin-like kinase enhances dendritic remodeling and negatively regulates synapse maturation2013
Author(s)
Shin, E., Kashiwagi, Y., Kuriu, T., Iwasaki, H., Tanaka, T., Koizumi, H., Gleeson, J. G. and S. Okabe
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Journal Title
Nature Communications
Volume: 4
Issue: 1
Pages: 1440-1440
DOI
Related Report
Peer Reviewed
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