Functional significance of angiogenesis in the circumventricular organs

• Project/Area Number: 24500411
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: MIYATA Seiji 京都工芸繊維大学, 工芸科学研究科, 准教授 (30243124)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 血管新生 / 神経幹細胞 / 血液脳関門 / 血管透過性 / 血管基定膜 / タイトジャンクション / TRPV1 / TLR4 / ホメオスタシス / 脳 / 神経内分泌 / 脳室周囲器官 / 神経新生 / 神経分泌 / 内皮細胞 / ペリサイト / ペプチドホルモン分泌

Outline of Final Research Achievements

The circumventricular organs (CVOs) do not express tight junction proteins and hence lack blood-brain barrier (BBB) and possess high vascular permeability. The present study demonstrates the presence of continuous angiogenesis and neurogenesis in the CVOs of adult mice. Continuous angiogenesis and neurogenesis contributes to tissue reconstruction and plasticity. Moreover, the basement membrane and GFAP-positive neural stem cells function as barrier instead of usual BBB. GFAP-positive neural stem cells detect blood-derived information via sensory protein TRPV1 and TLR4 and thereafter send information to adjacent neurons. Thus, the CVOs the dynamic brain regions accompanied with angiogenesis and neurogenesis.

Research Products

• [Journal Article] Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain (2015)
  • Author(s): Nakano, Y, Furube, E, Morita, S, Wanaka, A, Nakashima, T, Miyata, S
  • Journal Title: Journal of Neuroimmunology Volume: 278 Pages: 144-158
  • DOI: 10.1016/j.jneuroim.2014.12.013
  • Peer Reviewed
• [Journal Article] Vascular endothelial growth factor-dependent angiogenesis and dynamic vascular plasticity in the sensory circumventricular organs of adult mouse brain (2015)
  • Author(s): Morita, S, Furube, E, Mannari, T, Okuda, H, Tatsumi, K, Wanaka, A, Miyata, S
  • Journal Title: Cell and Tissue Research Volume: 359 Issue: 3 Pages: 865-884
  • DOI: 10.1007/s00441-014-2080-9
  • Peer Reviewed
• [Journal Article] Characterization of neural stem cells and their progeny in the sensory circumventricular organs of adult mouse. (2015)
  • Author(s): Furube, E, Morita, M., Miyata, S
  • Journal Title: Cell and Tissue Resarch Volume: 0 Issue: 2 Pages: 0-0
  • DOI: 10.1007/s00441-015-2201-0
  • Peer Reviewed / Open Access
• [Journal Article] Heterogenous vascular permeability and alternative diffusion barrier in the sensory circumventricular organs of adult mouse brain (2015)
  • Author(s): Morita, S, Furube, E, Mannari, T, Okuda, H, Tatsumi, K, Wanaka, A, Miyata, S
  • Journal Title: Cell and Tissue Research Volume: 0 Issue: 2 Pages: 0-0
  • DOI: 10.1007/s00441-015-2207-7
  • Peer Reviewed / Open Access
• [Journal Article] Activity-dependent Notch signaling in the hypothalamo-neurohypophysial system of adult mouse brains (2014)
  • Author(s): Mannari, T, Miyata, S
  • Journal Title: Journal of Neuroendocrinology Volume: 26 Issue: 8 Pages: 497-509
  • DOI: 10.1111/jne.12172
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Antidepressant-induced vascular dynamics in the hippocampus of adult mouse brain. (2014)
  • Author(s): Mannari, T, Sawa, H, Furube, E, Fukushima, S, Nishikawa, K, Nakashima, T, Miyata, S
  • Journal Title: Cell and Tissue Research Volume: 358 Issue: 1 Pages: 43-55
  • DOI: 10.1007/s00441-014-1933-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] VEGF-dependent and PDGF-dependent dynamic neurovascular reconstruction in the neurohypophysis of adult mice. (2014)
  • Author(s): Furube, E, Tetsuya, M, Morita, S, Nishikawa, K, Yoshida, A, Miyata, S
  • Journal Title: Journal of Endocrinology Volume: 221 Issue: 1 Pages: 161-179
  • DOI: 10.1530/joe-14-0075
  • Peer Reviewed
• [Journal Article] Structural characterization and subcellular localization of Drosophila organic solute carrier partner 1. (2014)
  • Author(s): Tho Huu, N, Yoshida, Umegawachi, T, Miyata, S, Yamaguchi, M
  • Journal Title: BMC Biochemistry Volume: 15 Issue: 1 Pages: 11-11
  • DOI: 10.1186/1471-2091-15-11
  • Peer Reviewed / Open Access
• [Journal Article] Different expression of alpha- synuclein in hippocampal neurons. (2014)
  • Author(s): Taguchi, K, Watanabe, Y, Tsujimura, A, Tatebe, H, Miyata, S, Tokuda, T, Mizuno, T, Tanaka, M
  • Journal Title: PLos One Volume: 9 Issue: 2 Pages: e89327-e89327
  • DOI: 10.1371/journal.pone.0089327
  • Peer Reviewed / Open Access
• [Journal Article] VEGF-dependent continuous angiogenesis in the medianeminence of adult mice (2013)
  • Author(s): Shoko Morita, Shinri Ukai,Seiji Miyata
  • Journal Title: European Journal of Neuroscience Volume: 37 Issue: 4 Pages: 508-518
  • DOI: 10.1111/ejn.12047
  • Peer Reviewed
• [Journal Article] Synaptic localization of GAP-43 in cultured hippocampal neurons during synaptogenesis. (2013)
  • Author(s): Morita S., Miyata S.
  • Journal Title: Cell Biochemistry and Function Volume: 31 Pages: 400-411
  • Peer Reviewed
• [Journal Article] Accessibility of low-molecular-mass molecules to the median eminence and arcuate hypothalamic nucleus of adult mouse. (2013)
  • Author(s): Morita S., Miyata S.
  • Journal Title: Cell Biochemistry and Function Volume: 31 Pages: 668-677
  • Peer Reviewed
• [Journal Article] Neurogenesis in the circumventricular organs of adult mouse brains. (2013)
  • Author(s): Hourai A., Miyata S.
  • Journal Title: Journal of Neuroscience Research Volume: 91 Issue: 6 Pages: 757-770
  • DOI: 10.1002/jnr.23206
  • Peer Reviewed
• [Journal Article] Astrocytic TRPV1 ion channels detect blood-borne signals in the sensory circumventricular organs of adult mouse brains. (2013)
  • Author(s): Mannari T, Morita S, Furube E, Tominaga M, Miyata S.
  • Journal Title: Glia Volume: 61 Issue: 6 Pages: 957-971
  • DOI: 10.1002/glia.22488
  • Peer Reviewed
• [Journal Article] Changes in pericytic expression of NG2 and PDGFRB and vascular permeability in the sensory circumventricular organs of adult mouse by osmotic stimulation (2013)
  • Author(s): Morita S, Hourai A, Miyata S
  • Journal Title: Cell Biochem Funct Volume: (Epub ahead of print) Issue: 1 Pages: 51-61
  • DOI: 10.1002/cbf.2971
  • Peer Reviewed
• [Journal Article] Overexpression of IgLON cell adhesion molecules changesproliferation and cell size of cortical astrocytes (2013)
  • Author(s): Chiaki Sugimoto, Shoko Morita, Seiji Miyata
  • Journal Title: Cell Biochemistry and Function Volume: 30 Issue: 5 Pages: 400-405
  • DOI: 10.1002/cbf.2813
  • Peer Reviewed
• [Journal Article] Synaptic localization of growth-associated protein 43 in culturedhippocampal neurons during synaptogenesis (2013)
  • Author(s): Shoko Morita, Seiji Miyata
  • Journal Title: Cell Biochemistry and Function Volume: (印刷中) Issue: 5 Pages: 400-411
  • DOI: 10.1002/cbf.2914
  • Peer Reviewed
• [Journal Article] Accessibility of low-molecular-mass molecules to the median eminence and arcuate hypothalamic nucleus of adult mouse (2013)
  • Author(s): Shoko Morita, Seiji Miyata
  • Journal Title: Cell Biochemistry and Function Volume: (印刷中) Issue: 8 Pages: 668-677
  • DOI: 10.1002/cbf.2953
  • Peer Reviewed
• [Journal Article] Different vascular permeability between the sensory and secretory circumventricular organs of adult mouse brain (2012)
  • Author(s): Morita S, Miyata S
  • Journal Title: Cell and Tissue Research Volume: 349 Issue: 2 Pages: 589-603
  • DOI: 10.1007/s00441-012-1421-9
  • Peer Reviewed
• [Presentation] 海馬神経線維が走行する脳弓におけるオリゴデンドロジェネシス (2015)
  • Author(s): 宮田清司、福島翔平、小野勝彦、竹林浩昌、中島敏博
  • Organizer: 第120回 日本解剖学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-23
• [Presentation] 血管新生による下垂体後葉血管ネットワーク構築変化の神経内分泌における意義 (2015)
  • Author(s): 古部瑛莉子、 萬成哲也、 森田晶子、中島敏博、宮田清司
  • Organizer: 第120回 日本解剖学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-22
• [Presentation] 成体マウスの下垂体後葉における血管新生 (2014)
  • Author(s): 古部瑛莉子、萬成哲也、森田晶子、宮田清司
  • Organizer: 第90回 近畿支部学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-29
• [Presentation] VEGF と PDGF に依存した下垂体後葉の血管新生と終末-血管のダイナミクス (2014)
  • Author(s): 古部瑛莉子、 萬成哲也、 森田晶子、宮田清司
  • Organizer: 第41回 日本神経内分泌学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-31
• [Presentation] LPS刺激による脳室周囲器官神経幹細胞とミクログリアの増殖変化 (2014)
  • Author(s): 古部瑛莉子、 森田光博、宮田清司
  • Organizer: 第37回 日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-12
• [Presentation] PDGFシグナルによる血管系のペリサイトと神経分泌制御 (2014)
  • Author(s): 西川 和範、宮田 清司
  • Organizer: 第37回 日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-12
• [Presentation] 脳室周囲器官のアストロサイトTRPV1による血液情報感知機構 (2013)
  • Author(s): 萬成哲也、森田昌子、富永真琴、宮田清司
  • Organizer: 第３６回日本神経科学大会
  • Place of Presentation: 国立京都国際会議場
• [Presentation] 成体マウス脳弓におけるオリゴデンドロジェネシス；海馬神経交連・投射繊維のミエリン化制御 (2013)
  • Author(s): 福島翔平、西川和範、小野勝彦、竹林浩秀、宮田清司
  • Organizer: 第３６回日本神経科学大会
  • Place of Presentation: 国立京都国際会議場
• [Presentation] 成体マウスの脳室周囲器官における血管新生因子VEGFとNocthシグナリング (2013)
  • Author(s): 鵜飼真璃、森田昌子、宮田清司
  • Organizer: 第３６回日本神経科学大会
  • Place of Presentation: 国立京都国際会議場
• [Presentation] 感知系脳室周囲器官のダイナミックな血管構築変化とアストロサイトバリア：血中情報感知システムにおける役割 (2013)
  • Author(s): 森田昌子、鵜飼真璃、奥田洋明、辰巳晃子、和中明生、宮田清司
  • Organizer: 第３６回日本神経科学大会
  • Place of Presentation: 国立京都国際会議場
• [Presentation] Lipopolysaccharide刺激による脳室周囲器官のastrocyte活性化 (2013)
  • Author(s): 中野洋輔、宮田清司
  • Organizer: 第３６回日本神経科学大会
  • Place of Presentation: 国立京都国際会議場
• [Presentation] 神経分泌における血管系ダイナミックスの意義 (2013)
  • Author(s): 宮田清司、森田昌子
  • Organizer: 第３６回日本神経科学大会
  • Place of Presentation: 国立京都国際会議場
• [Presentation] 成体マウスの脳室周囲器官における神経幹前駆細胞 (2013)
  • Author(s): 古部瑛莉子、蓬莱敦、宮田清司
  • Organizer: 第３６回日本神経科学大会
  • Place of Presentation: 国立京都国際会議場
• [Presentation] 成体マウスの海馬における血管新生 (2013)
  • Author(s): 澤速人、萬成哲也、宮田清司
  • Organizer: 第３６回日本神経科学大会
  • Place of Presentation: 国立京都国際会議場
• [Presentation] LPS刺激による脳室周囲器官アストロサイトのSTAT3核内移行
  • Author(s): 中野洋輔・宮田清司
  • Organizer: 第８９回日本解剖学会近畿支部学術集会
  • Place of Presentation: 奈良先端科学技術大学
• [Presentation] 成体マウスの脳弓におけるオリゴデンドロジェネシス
  • Author(s): 福島翔平・宮田清司
  • Organizer: 第８９回日本解剖学会近畿支部学術集会
  • Place of Presentation: 奈良先端科学技術大学
• [Presentation] 脳室周囲器官におけるアストロサイトTRPV1を介した血液由来情報の受容機構
  • Author(s): 萬成 哲也・宮田清司
  • Organizer: 第８９回日本解剖学会近畿支部学術集会
  • Place of Presentation: 奈良先端科学技術大学
• [Remarks] 研究室ホームページ
  • URL: http://www.cis.kit.ac.jp/~smiyata/top.htm
• [Remarks] SEiji Miyata Research Map
  • URL: http://researchmap.jp/read0013597/
• [Remarks] Seiji Miyata Research Gate
  • URL: https://www.researchgate.net/profile/Seiji_Miyata?ev=hdr_xprf
• [Remarks] http://www.cis.kit.ac.jp/~smiyata/top.htm