| Project/Area Number |
24500421
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAMMA Hiroshi 杏林大学, 教授 (10195191)
YAZAWA Takuya 千葉大学, 医学(系)研究科, 准教授 (50251054)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 進行性多巣性白質脳症 / JCウイルス / 細胞周期 / PML-NBs / DNA複製 / 細胞変性 / 細胞腫瘍化 / 病理学 / 脳腫瘍 |
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| Outline of Final Research Achievements |
Progressive multifocal leukoencephalopathy is a fatal demyelinating disorder caused by JC virus infection. Histopathologically, infected oligodendroglia-like cells display two distinctive inclusion patterns: full inclusions in which progeny virions occupy throughout the enlarged nucleus and dot-shaped inclusions in which virions are clustered at subnuclear domains called promyelocytic leukemia nuclear bodies (PML-NBs). In this study, we clarified that nuclei of infected oligodendrocytes enlarge with cell cycle transition from S to G2-like state. PML-NBs also enlarge during this process, and the virus re-produce progenies. Eventually, PML-NBs are dispersed by propagating viruses. Therefore PML-NBs serve a scaffolding role for viral progeny production however it remains unclear why the virus-infected oligodendrocytes undergo cell death but not mitosis, after cell cycle transition from S to G2.
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