Research Project
Grant-in-Aid for Scientific Research (C)
Neuromelanin (NM) occurs in dopaminergic neurons of the substantia nigra (SN) and in noradrenergic neurons of the locus coeruleus (LC). HPLC analysis after HI hydrolysis of natural NM detected AHPEA, AHPAA and AHEB, which confirmed that SN-NM and LC-NM contain melanin derived not only from DA and NE, but also from several other catecholic metabolites, such as DOPA, DOPAC, DOMA, DOPE and DOPEG, in addition to the corresponding Cys-derivatives in varying degrees. However, HI hydrolysis showed that LC-NM produced the same degradation products as were detected in SN-NM. Thus, we needed to develop a new chemical detection method to validate the existence of NE in LC-NM. HCl hydrolysis of LC-NM in the presence of thioglycolic acid yields new products arising from substitution of the hydroxyl group by thioglycolic acid at the benzyl position of NE and cysteinyl-NE. This is the first chemical evidence showing that NE and cysteinyl-NE are incorporated into LC-NM.
All 2014 2013 2012
All Journal Article (29 results) (of which Peer Reviewed: 29 results, Open Access: 1 results, Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Invited: 1 results)
Molecules
Volume: 19 Issue: 6 Pages: 8039-8050
10.3390/molecules19068039
J. Dermatol. Sci.
Volume: 73 Issue: 3 Pages: 76-87
10.1016/j.jdermsci.2013.11.006
Behavioral Ecology
Volume: 25 Issue: 1 Pages: 76-87
10.1093/beheco/art088
J. Evol. Biol.
Volume: 27 Issue: 3 Pages: 660-666
10.1111/jeb.12328
J Dermatol
Volume: 41 Issue: 4 Pages: 296-302
10.1111/1346-8138.12432
Bioorg. Med. Chem. Lett.
Volume: 24 Issue: 9 Pages: 2150-2154
10.1016/j.bmcl.2014.03.031
Anal. Biochem.
Volume: 434 Issue: 2 Pages: 221-225
10.1016/j.ab.2012.12.005
Pigment Cell Melanoma Res
Volume: 26 Issue: 3 Pages: 357-366
10.1111/pcmr.12078
Plos One
Volume: 8 Issue: 2 Pages: e58024-e58024
10.1371/journal.pone.0058024
Volume: 8 Issue: 4 Pages: e60426-e60426
10.1371/journal.pone.0060426
J. Skin Cancer
Volume: 2013 Pages: 174-180
10.1155/2013/742925
Spectrochimica Acta Part A Molecular and Biomolecular Spctroscopy
Volume: 110 Pages: 55-59
10.1016/j.saa.2013.03.027
Pigment Cell Melanoma Res.
Volume: 26 Issue: 5 Pages: 723-726
10.1111/pcmr.12118
Volume: 26 Issue: 5 Pages: 616-633
10.1111/pcmr.12121
Biol. J. the Linnean Soc.
Volume: 109 Issue: 3 Pages: 562-573
10.1111/bij.12078
Volume: 8 Issue: 7 Pages: e69346-e69346
10.1371/journal.pone.0069346
Pigment Cell melanoma Res.
Volume: 26 Pages: 917-923
Volume: 26 Issue: 6 Pages: 819-825
10.1111/pcmr.12137
J. Herpetol.
Volume: 47 Issue: 2 Pages: 258-261
10.1670/12-028
Small Ruminant Res.
Volume: 114 Issue: 2-3 Pages: 240-246
10.1016/j.smallrumres.2013.07.004
Org Geochem
Volume: 64 Pages: 29-37
10.1016/j.orggeochem.2013.09.002
Zool Sci
Volume: 31 Issue: 2 Pages: 53-63
10.2108/zsj.31.53
40019989060
Aesthertic Dermatology
Volume: 23 Pages: 63-73
Visual Dermatology
Volume: 12 Pages: 601-607
Volume: 26 Pages: 357-366
Volume: 25 Pages: 792-803
PLoS One
Volume: 7 Issue: 11 Pages: e48490-e48490
10.1371/journal.pone.0048490
Volume: 25 Pages: 434-445
Proc. Natl. Acad. Sci. USA
Volume: 109 Pages: 10218-10223