| Project/Area Number |
24500454
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Institute for Developmental Research, Aichi Human Service Center |
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Principal Investigator |
KOUCHI Zen 愛知県心身障害者コロニー発達障害研究所, 病理学部, 研究員 (70322485)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOKAWA Masanori 愛知県心身障害者コロニー・発達障害研究所, 病理学部, 部長(兼任) (00127135)
SHIMADA Atsuyoshi 愛知県心身障害者コロニー・発達障害研究所, 病理学部, 室長(兼任) (50311444)
ENOKIDO Yasushi 愛知県心身障害者コロニー・発達障害研究所, 病理学部, 室長 (90263326)
CHIBA Yoichi 元愛知県心身障害者コロニー・発達障害研究所, 病理学部, 主任研究員 (30372113)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | microglia / LPS / cytokine / monoacylglycerol lipase / ABHD12 / ミクログリア / モノアシルグリセロールリパーゼ / MAGL / サイトカイン / 低酸素性虚血性脳症 / 炎症性サイトカイン / 貪食 / 脂質分解 |
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| Outline of Final Research Achievements |
It has been known that monoacylglycerol lipase (MAGL) is involved in wiring process of neural network in brain development, however, its pathological function in inflammatory condition remains unknown. We found that MAGL transcription was downregulated in neonatal brain subjected to hypoxic/ischemic stress and primary microglia treated with lipopolysaccharide (LPS). Microglial MAGL was transcriptionally regulated by Stat6 and its protein was rapidly degraded by proteasome in steady state. LPS treatment inhibited its degradation, resulting in its stabilization in microglia. Intrinsic MAGL was not prerequisite for LPS-dependent induction of several inflammatory cytokines such as IL6 in normoxic or hypoxic conditions, whereas it promoted phagocytosis mediated by Fcγ receptor in microglia.
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