| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Ca2+ signals are highly regulated in a spatiotemporal manner in numerous cellular physiological events. Here, I engineered blue light-activated Ca2+ channel switch (BACCS), as an optogenetic tool for manipulating Ca2+ signals. Ca2+ influx was induced in various cell types expressing BACCS. Local Ca2+ signals were generated in neurites of mouse hippocampal neurons by local exposure to light. In the mouse olfactory system, neurons expressing BACCS showed electrophysiological responses in response to light, suggesting that it is applicable to in vivo studies. Thus, BACCSs will be a useful optogenetic tool for controlling intracellular Ca2+ both in vitro and in vivo.
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