Analysis of the action of oxytocin at synapse level in regulating nociceptive transmission in rat spinal dorsal horn
| Project/Area Number |
24500461
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Saga University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Tsugumi 佐賀大学, 医学部, 准教授 (70336139)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | オキシトシン / 鎮痛 / 脊髄後角 / 興奮性シナプス伝達 / 抑制性シナプス伝達 / 脱分極 / ラット / パッチクランプ法 / オキシトシン受容体 / ホスホリパーゼC / IP3誘起Ca2+放出 |
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| Outline of Final Research Achievements |
We examined how oxytocin affects synaptic transmission in substantia gelatinosa (SG) neurons by applying the patch-clamp technique to the SG neurons of adult rat spinal cord slices. Bath-applied oxytocin unaffected excitatory transmission while producing an inward current at -70 mV and enhancing spontaneous inhibitory transmission. These activities were mimicked by oxytocin-receptor agonist. The oxytocin current was inhibited by oxytocin-receptor antagonist, U-73122, 2-aminoethoxydiphenyl borate but not dantrolene, chelerythrine, dibutyryl cyclic-AMP and tetrodotoxin, while the spontaneous inhibitory transmission enhancement was depressed by tetrodotoxin. It is concluded that oxytocin produces a membrane depolarization mediated by oxytocin receptors, which increases neuronal activity, resulting in the enhancement of inhibitory transmission, a possible mechanism for antinociception. This depolarization is possibly mediated by phospholipase C and IP3-induced Ca2+-release.
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Report
(4 results)
Research Products
(116 results)
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[Presentation] Enhanced pain behavior observed in mice lacking interleukin-27.2014
Author(s)
Toshiharu Yasaka, Tomoko Sasaguri, Yuzo Murata, Hiromitsu Hara, Asako Ishikawa, Tsugumi Fujita, Eiichi Kumamoto, Sadahiko Masuko, Naomi Hirakawa, Hiroki Yoshida
Organizer
Neuroscience 2014 - Society for Neuroscience the 44th Annual Meeting
Place of Presentation
The Walter E. Convention Center (Washington, D.C., USA)
Year and Date
2014-11-15 – 2014-11-19
Related Report
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[Presentation] Inhibition by various aroma-oil chemicals of compound action potentials in the frog sciatic nerve.2013
Author(s)
Sena Ohtsubo, Akitomo Matsushita, Tsugumi Fujita, Hiroki Kawasaki, Chang-Yu Jiang, Qing-Tian Luo, Qin Kang, Sadahiko Masuko, Eiichi Kumamoto
Organizer
Neuroscience 2013; Society for Neuroscience the 43rd Annual Meeting
Place of Presentation
The San Diego Convention Center (San Diego, CA, USA)
Related Report
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[Presentation] 1,8-および1,4-シネオールは成熟ラット脊髄膠様質ニューロンのグルタミン酸作動性の自発性興奮性シナプス伝達を促進する.2013
Author(s)
藤田亜美, 徐 年香, 蒋 昌宇, 羅 清甜, 康 欽, 八坂敏一, 大坪瀬奈, 松下晋大, 熊本栄一
Organizer
第36回日本神経科学大会
Place of Presentation
国立京都国際会館 (京都府京都市)
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