The study for cerebellar cortical information processing using functional imaging and three-dimensional reconstruction

• Project/Area Number: 24500476
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Fusional basic brain science
• Research Institution: The Institute of Physical and Chemical Research (2013-2014); Saitama University (2012)
• Principal Investigator: MICHIKAWA TAKAYUKI 独立行政法人理化学研究所, 光量子工学研究領域, 研究員 (90282516)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: カルシウムイメージング / 国際情報交換

Outline of Final Research Achievements

Adenovirus can be used to express genetically encoded calcium indicators into specific types of neurons, such as cerebellar Purkinje cells. In vivo two-photon imaging of a large population of Purkinje cells will be useful to identify functional circuits that participate in information processing in cerebellar cortex. In this study, I have established experimental procedures and deconvolution-based data analysis methods suitable for inferring single complex spike firings of Purkinje cells from two-photon calcium imaging data acquired from living mice expressing a genetically encoded ratiometric calcium indicator, yellow cameleon 2.60.

Research Products

• [Journal Article] Phospholipase C-β1 and β4 contribute to non-genetic cell-to-cell variability in histamine-induced calcium signals in HeLa cells (2014)
  • Author(s): Ishida S, Matsu-Ura T, Fukami K, Michikawa T, Mikoshiba K.
  • Journal Title: PloS One Volume: 9
  • DOI: 10.1371/journal.pone.0086410
  • Peer Reviewed / Open Access
• [Journal Article] Optimal microscopic systems for long-term imaging of intracellular calcium using a ratiometric genetically-encoded calcium indicator. (2014)
  • Author(s): Miyamoto A, Bannai H, Michikawa T, Mikoshiba K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 434 Pages: 252-257
  • DOI: 10.1016/j.bbrc.2013.02.112
  • Peer Reviewed
• [Journal Article] Cooperative and stochastic calcium releases from multiple calcium puffsites generate calcium microdomains in intact HeLa cells. (2012)
  • Author(s): Nakamura H, Bannai H, Inoue T, Michikawa T, Sano M, Mikoshiba K.
  • Journal Title: Journal of Biological Chemistry Volume: 287 Pages: 24563-24572
  • DOI: 10.1074/jbc.m111.311399
  • Peer Reviewed
• [Presentation] Optical recording of neural activity using yellow cameleon 2.60-expressing mice (2014)
  • Author(s): Michikawa, T, Itohara, S., and Miyawaki, A,
  • Organizer: 理研シンポジウム：第２回「光量子工学研究」
  • Place of Presentation: 仙台市情報・産業プラザ 5階多目的ホール
  • Year and Date: 2014-11-25 – 2014-11-26
• [Presentation] Cell-type selective wide-field calcium imaging, combined Yellow Cameleon 2.60 Tg mice and macromicroscopy (2013)
  • Author(s): Kuroki, S., Tsutsui H., Michikawa T., Iwama M., Miyawaki A. and Itohara S.
  • Organizer: 日本神経科学会大会
  • Place of Presentation: 国立京都国際会館
• [Presentation] In vivo calcium dynamics in cerebellar Purkinje cell dendrites (2012)
  • Author(s): Michikawa, T., Miyawaki, A., Kakei, S., Hausser, M., Itohara, S. and Nakai, J.
  • Organizer: Society for Neuroscience
  • Place of Presentation: New Orleans, USA
• [Presentation] Cell-type selective wide-field calcium imaging, combined Yellow Cameleon 2.60 Tg mice and macromicroscopy (2012)
  • Author(s): Kuroki, S., Michikawa, T., Tsutsui, H., Manita, S., Shimozono, S., Murayama, M., Miyawaki, A. and Itohara, S
  • Organizer: Molecular and Cellular Cognition Society
  • Place of Presentation: New Orleans, USA
• [Presentation] Establishment of transgenic mice expressing calcium sensor Yellow Cameleon 2.60 and applications to analysis of the thalamocortical pathway (2012)
  • Author(s): Kuroki, S., Michikawa, T., Manita, S., Tsutsui, H., Shimozono, S., Murayama, M., Miyawaki, A. and Itohara, S
  • Organizer: 日本神経科学会
  • Place of Presentation: 名古屋