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Generation of knock out rat using mouse <-> rat chimera

Research Project

Project/Area Number 24500490
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionOsaka University

Principal Investigator

ISOTANI Ayako  大阪大学, 免疫学フロンティア研究センター, 特任准教授 (20444523)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords疾患モデル動物 / 遺伝子組換え / 異種キメラ / 遺伝子組み換え / モデル動物
Outline of Final Research Achievements

In a previous study, we produced mouse←rat chimera by injecting rat embryonic stem (ES) cells into mouse blastocysts. In this model, rat ES cells contributed to various organs, including spermatozoa, indicating potential utility for the production of a knockout rat. Hence, we established new rat ES cell lines to visualize rat spermatozoa using green fluorescent protein (GFP) from green body-green sperm (GBGS) doubly transgenic rats. Subsequently, we prepared a homologous recombined rat ES cell lines. When GBGS-rat ES cells were used to produce mouse←rat chimera, rat spermatozoa were easily detected with GFP in chimeric testes. Subsequently, the germline potential of rat spermatozoa in the chimera were assessed by intracytoplasmic injections.   Rat pups were generated from rat spermatozoa in chimeric testes, and normal germline potential of rat spermatozoa in the chimeric testes was demonstrated. This study provides a novel method for the production of knockout rat.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (3 results) Book (3 results) Remarks (8 results)

  • [Journal Article] Induction of Primordial Germ Cell-Like Cells From Mouse Embryonic Stem Cells by ERK Signal Inhibition.2014

    • Author(s)
      Kimura T, Kaga Y, Ohta H, Odamoto M, Sekita Y, Li K, Yamano N, Fujikawa K, Isotani A, Sasaki N, Toyoda M, Hayashi K, Okabe M, Shinohara T, Saitou M, Nakano T.
    • Journal Title

      Stem Cells

      Volume: 32 Issue: 10 Pages: 2668-2678

    • DOI

      10.1002/stem.1781

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.2013

    • Author(s)
      Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M.
    • Journal Title

      Scientific Reports

      Volume: 3: 3355 Issue: 1 Pages: 1-6

    • DOI

      10.1038/srep03355

    • NAID

      120006957396

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] SPACA1-deficient male mice are infertile with abnormally shaped sperm heads reminiscent of globozoospermia2012

    • Author(s)
      Fujihara Y, Satouh Y, Inoue N, Isotani A, Ikawa M, Okabe M
    • Journal Title

      Development

      Volume: 139 Issue: 19 Pages: 3583-9

    • DOI

      10.1242/dev.081778

    • NAID

      120006957401

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] マウス←ラットESキメラを介した遺伝子変異ラットの作出2015

    • Author(s)
      磯谷 綾子
    • Organizer
      第62回日本実験動物学会総会
    • Place of Presentation
      京都テルサ
    • Year and Date
      2015-05-28 – 2015-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] A trial to form an organ from ES cells --- a rat thymus in rat-nude mouse chimera.2012

    • Author(s)
      磯谷 綾子
    • Organizer
      第7回 研究所ネットワーク国際シンポジウム
    • Place of Presentation
      東北大学加齢医学研究所
    • Related Report
      2012 Research-status Report
  • [Presentation] マウスとラットの異種キメラの話2012

    • Author(s)
      磯谷 綾子
    • Organizer
      第5回生殖若手の会
    • Place of Presentation
      東京大学三崎臨海実験所
    • Related Report
      2012 Research-status Report
  • [Book] 実験医学2014

    • Author(s)
      磯谷綾子
    • Total Pages
      132
    • Publisher
      羊土社
    • Related Report
      2014 Annual Research Report
  • [Book] プログレッシブ 生命科学2014

    • Author(s)
      磯谷綾子、伊川正人
    • Total Pages
      312
    • Publisher
      南山堂
    • Related Report
      2014 Annual Research Report
  • [Book] 生命科学から創薬へのイノベーション2014

    • Author(s)
      磯谷綾子、伊川正人
    • Total Pages
      202
    • Publisher
      南山堂
    • Related Report
      2014 Annual Research Report
  • [Remarks] 自己紹介ページ

    • URL

      http://www.egr.biken.osaka-u.ac.jp/information/ayako_isotani.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 大阪大学微生物病研究所遺伝子機能解析学分野ホームページ

    • URL

      http://www.egr.biken.osaka-u.ac.jp/information/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 大阪大学微生物病研究所/免疫学フロンティア研究センター附属感染動物実験施設ホームページ

    • URL

      http://www.arcid.biken.osaka-u.ac.jp/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 大阪大学 微生物病研究所 遺伝子機能解析分野 ホームページ

    • URL

      http://www.egr.biken.osaka-u.ac.jp/information/member.html

    • Related Report
      2013 Research-status Report
  • [Remarks] 個人ホームページ

    • URL

      http://www.egr.biken.osaka-u.ac.jp/information/ayako_isotani.html

    • Related Report
      2013 Research-status Report
  • [Remarks] 遺伝子機能解析分野のホームページ

    • URL

      http://www.egr.biken.osaka-u.ac.jp/information/index.html

    • Related Report
      2012 Research-status Report
  • [Remarks] 感染動物実験施設のホームページ

    • URL

      http://www.arcid.biken.osaka-u.ac.jp/school/

    • Related Report
      2012 Research-status Report
  • [Remarks] 自己紹介 -personal data-

    • URL

      http://www.egr.biken.osaka-u.ac.jp/information/ayako_isotani.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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