| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Four transgenic (Tg) mice were developed by injection of constructs involving DTR expression cassette regulated by promoters of neutrophil-specific genes. Upon genotyping and RT-PCR for peripheral blood, it was demonstrated that DTR expression levels were not only dependent on promoters used but also different among individual mice. In addition, these phenotypes were inheritable. The Tg mice showing ~50% reduction of neutrophils were obtained following DT administration condition examination. In order to investigate the role of neutrophils by using these mice, mice models in which upper and lower airway-specific allergic inflammation was developed were established.
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