Development of inducible and constitutive neutrophil-deficient mice for analyzing allergic inflammation
| Project/Area Number |
24500501
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
KAMINUMA Osamu 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (80342921)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUOKA Kunie 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (40291158)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 実験動物学 / 疾患モデル |
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| Outline of Final Research Achievements |
Four transgenic (Tg) mice were developed by injection of constructs involving DTR expression cassette regulated by promoters of neutrophil-specific genes. Upon genotyping and RT-PCR for peripheral blood, it was demonstrated that DTR expression levels were not only dependent on promoters used but also different among individual mice. In addition, these phenotypes were inheritable. The Tg mice showing ~50% reduction of neutrophils were obtained following DT administration condition examination. In order to investigate the role of neutrophils by using these mice, mice models in which upper and lower airway-specific allergic inflammation was developed were established.
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Report
(4 results)
Research Products
(18 results)
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[Presentation] Pharmacological characterization of T cell-induce bronchoconstriction in the mice.2015
Author(s)
Mori A, Kouyama S, Yamaguchi M, Iijima Y, Ohtomo-Abe A, Hayashi H, Watarai K, Mitsui C, Oshikata C, Sekiya K, Tsuburai T, Maeda Y, Ohtomo M, Fukutomi Y, Taniguchi M, Akiyama K, Ohtomo T, Kaminuma O.
Organizer
2015 American Academy of Allergy, Asthma and Immunology annual meeting
Place of Presentation
Huston, USA
Year and Date
2015-02-20 – 2015-02-24
Related Report
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[Presentation] Development and treatment of steroid resistant asthma model by adoptive transfer of murine helper t cell clones.2014
Author(s)
Mori A, Kouyama S, Yamaguchi M, Iijima Y, Ohtomo A, Ohtomo T, Itoh J, Hayashi H, Watarai K, Mitsui C, Oshikata C, Fukutomi Y, Sekiya K, Tsuburai T, Maeda Y, Ohtomo M, Taniguchi M, Akiyama K, Kaminuma O.
Organizer
WAO International Scientific Conference 2014
Place of Presentation
Rio de Janeiro, Brazil
Year and Date
2014-12-06 – 2014-12-09
Related Report
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