Mechanism of cancer cell apoptosis under low voltage electric pulses
| Project/Area Number |
24500524
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Okayama University of Science |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 低電圧パルス / カスパーゼ / 細胞内カルシウム / アポトーシス / Apoptosis / Ca / Electric pulses / 低電圧電気パルス |
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| Outline of Final Research Achievements |
High-intensity electrochemotherapy has been investigated as a novel anticancer therapy. However, the application of high-intensity electric fields puts great stress on patients. Therefore, cell death induced by a milder electric field would be a favorable alternative to high-voltage electrochemotherapy. In general, mild electric fields appear to induce less cell damage and greater cell survival than high-intensity fields. Research into the cellular effects of exposure to Low voltage electric pulses (LVEPs) is useful for cancer research.
We examined the role of caspases activities and intracellular Ca2+ in the subsequent cellular response. As a result, we showed that a mild electric field could be a favorable alternative to high-voltage electrochemotherapy. Chemotherapy is generally effective in aerobic condition. Therefore, we studied oxygen micro bubble technique to make cancer tissue aerobic condition. We propose new mild electrochemotherapy under aerobic condition.
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Report
(4 results)
Research Products
(2 results)
