| Project/Area Number |
24500625
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Kansai University of Welfare Sciences |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MORI Yoshiaki 関西福祉科学大学, 保健医療学部, 教授 (70268192)
YAMAJI Junko 関西福祉科学大学, 健康福祉学部, 准教授 (40340559)
YAMAMOTO Maki 関西福祉科学大学, 保健医療学部, 教授 (60240123)
HIROSHIMA Reiko 関西福祉科学大学, 保健医療学部, 准教授 (40404777)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ミオシン重鎖タイプI / インターロイキン-6 / 熱ショック蛋白70 / カルシニューリン / 熱ショック蛋白質70 / IL-6 / La3+ / クロロゲン酸 / IGF-1 / ミオシン重鎖 / 熱ショックタンパク / 骨格筋細胞 / 骨格筋 / HSP-70 |
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| Outline of Final Research Achievements |
We examined effects of IL-6 or La3+ on MHC I, HSP70, and IL-6 mRNA expression level using real-time PCR method in C2C12 skeletal muscle cells. First, we examined the effect of IL-6 on MHC I, HSP70, and IL-6 mRNA expression levels. These mRNA levels were significantly increased by the incubation with IL-6. Second, we examined the effect of La3+ on MHC I, HSP70, and IL-6 mRNA expression levels. These mRNA levels were significantly increased by the incubation with La3+. The effects of La3+ on these mRNA levels are considered as a result of calcineurin activation, because it was well known that La3+ stimulate the activity of calcineurin. La3+-induced upregulation of these mRNA levels were significantly attenuated by the application of calcineurin inhibitor, cyclosporin A. These results indicate that IL-6- and La3+/calcineurin-mediated mechanisms contribute to the upregulation of MHC I mRNA levels in C2C12 cells.
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