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The improving effect of dialysis patients' physical activity endurance using the glycogen synthesis efficiency in hepatocytes caused by citrate-containing dialysate.

Research Project

Project/Area Number 24500662
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Rehabilitation science/Welfare engineering
Research InstitutionFujita Health University

Principal Investigator

OHASHI Atsushi  藤田保健衛生大学, 医療科学部, 准教授 (30310585)

Co-Investigator(Kenkyū-buntansha) NAKAI Shigeru  藤田保健衛生大学, 医療科学部, 教授 (20345896)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords血液透析 / 酢酸添加透析液 / クエン酸添加透析液 / 生体適合性 / グリコーゲン産生 / エネルギー代謝 / ヒト肝細胞 / グリコーゲン合成 / 持久力 / 肝臓細胞 / 骨格筋細胞 / グリコーゲン代謝 / 抗酸化作用 / 肝癌細胞 / グリコーゲン
Outline of Final Research Achievements

Citrate-containing dialysate (CD) has been considered to cause post-dialysis fatigue to a lesser extent than acetate-containing dialysate (AD). The aim of this study was to validate from a molecular biological perspective a robust glycogen synthesis effect by qRT-PCR. Normal human hepatocytes (HHpC) were cultured with EMEM and AD (AD/EM) or CD (CD/EM) for 4 h. The cells were then lysed, and the glycogen level, as well as the expression levels of glycogen synthase (GYS2), was measured. The glycogen level in HHpC incubated in CD/EM was 5.8 ± 0.8 μg/g, which was significantly higher than 5.1 ± 0.9 observed when incubated in AD/EM. The expression levels of GYS2 in CD/EM was 1.2 fold of that in AD/EM. In this study, it was found that increased glycogenesis capacity of hepatocytes cultured in CD, compared with AD, causes less fatigue feeling after dialysis, which is due to an increased energy level during dialysis in the presence of CD, and contributes to the energy supply after dialysis.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report

Research Products

(5 results)

All 2015 2014 2013 2012

All Presentation

  • [Presentation] クエン酸添加透析液の正常ヒト肝細胞に対するグリコーゲン装填能の検討2015

    • Author(s)
      大橋篤、堀秀生、川口和紀、中井滋、北口暢哉、比企能之、富田亮、長谷川みどり、湯澤由紀夫
    • Organizer
      第31回日本医工学治療学会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 酢酸およびクエン酸添加透析液のヒト骨格筋細胞に対するグリコーゲン代謝への影響2014

    • Author(s)
      大橋篤、堀秀生、川口和紀、中井滋、北口暢哉、比企能之、富田亮、長谷川みどり、湯澤由紀夫,
    • Organizer
      第59回日本透析医学会
    • Place of Presentation
      神戸ポートピアホテル
    • Year and Date
      2014-06-13 – 2014-06-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Vitamin-E-bonded polysulfone membrane prevent carbonylation of serum albumin and improves health-related quality of life in hemodialysis patients.2013

    • Author(s)
      Atsushi Ohashi  Sigeru Nakai
    • Organizer
      31th Annual Meeting of the International Society of Blood Purification
    • Place of Presentation
      Bologna (Italy)
    • Related Report
      2013 Research-status Report
  • [Presentation] 抗酸化透析療法よる血漿成分の酸化ストレスとSF36を用いたQOLへの影響.2013

    • Author(s)
      大橋篤、中井滋、
    • Organizer
      第58回日本透析医学会学術集会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 維持透析患者におけるビタミンE被覆膜の抗酸化効果の評価2012

    • Author(s)
      大橋篤
    • Organizer
      日本透析医学会
    • Place of Presentation
      札幌芸術文化の館(札幌)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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