The improving effect of dialysis patients' physical activity endurance using the glycogen synthesis efficiency in hepatocytes caused by citrate-containing dialysate.
| Project/Area Number |
24500662
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Fujita Health University |
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Principal Investigator |
OHASHI Atsushi 藤田保健衛生大学, 医療科学部, 准教授 (30310585)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAI Shigeru 藤田保健衛生大学, 医療科学部, 教授 (20345896)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 血液透析 / 酢酸添加透析液 / クエン酸添加透析液 / 生体適合性 / グリコーゲン産生 / エネルギー代謝 / ヒト肝細胞 / グリコーゲン合成 / 持久力 / 肝臓細胞 / 骨格筋細胞 / グリコーゲン代謝 / 抗酸化作用 / 肝癌細胞 / グリコーゲン |
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| Outline of Final Research Achievements |
Citrate-containing dialysate (CD) has been considered to cause post-dialysis fatigue to a lesser extent than acetate-containing dialysate (AD). The aim of this study was to validate from a molecular biological perspective a robust glycogen synthesis effect by qRT-PCR. Normal human hepatocytes (HHpC) were cultured with EMEM and AD (AD/EM) or CD (CD/EM) for 4 h. The cells were then lysed, and the glycogen level, as well as the expression levels of glycogen synthase (GYS2), was measured. The glycogen level in HHpC incubated in CD/EM was 5.8 ± 0.8 μg/g, which was significantly higher than 5.1 ± 0.9 observed when incubated in AD/EM. The expression levels of GYS2 in CD/EM was 1.2 fold of that in AD/EM. In this study, it was found that increased glycogenesis capacity of hepatocytes cultured in CD, compared with AD, causes less fatigue feeling after dialysis, which is due to an increased energy level during dialysis in the presence of CD, and contributes to the energy supply after dialysis.
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Report
(4 results)
Research Products
(5 results)
