The roles of MAPkinase to the differentiation of regenerating fiber phenotype and the effects of heat stress

• Project/Area Number: 24500791
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Sports science
• Research Institution: Kumamoto University
• Principal Investigator: OISHI Yasuharu 熊本大学, 教育学部, 教授 (10203704)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: マップキナーゼ / ヒラメ筋 / 筋線維組成 / 速筋線維 / 遅筋線維 / ミオシン重鎖 / ラット / 再生筋線維 / サイクロスポリンA / カルシニューリン / ブピバカイン / 熱ショックタンパク質 / 熱ストレス / 高齢ラット / 筋線維横断面積 / 筋衛星細胞 / MyoD / Pax7

Outline of Final Research Achievements

The relation between the skeletal muscle fiber phenotype and mitogen-activated protein kinase (MAPK) was investigated. Adult rat soleus muscle which contains the highest of slow fibers expressed lowest MAPK protein, while the highest level of MAPK protein was observed at the superficial portion of the gastrocnemius muscle which contains the highest of fast type fibers. In addition, the percentage of slow type fibers increase in an anti-gravity rat soleus muscle with growing and maturation, at those period MAPK protein gradually decreased.
These results may suggest that MAPK protein is relating to the expression of fast phenotype of rat skeletal muscle fibers.

Research Products

• [Journal Article] Heat stress effects on the myosin heavy chain phenotype of rat soleus fibers during the early stages of regeneration (2015)
  • Author(s): Yasuharu Oishi, Roland R. Roy, Tomonori Ogata, Yoshinobu Ohira
  • Journal Title: Muscle and Nerve Volume: 51 Pages: 900-910
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of heat stress on regenerating fibers of aged rat soleus muscle. (2013)
  • Author(s): Oishi Y
  • Journal Title: Bulletin of Faculty of Education, Kumamoto University. Volume: 62 Pages: 209-212
  • NAID: 110009659880
• [Journal Article] Skeletal muscle fiber plasticity: heat shock proteins and satellite cell activation (2012)
  • Author(s): Yasuharu Oishi and Tomonori Ogata
  • Journal Title: J PhysFitness Sports Med Volume: 1(3) Pages: 473-478
  • Peer Reviewed
• [Presentation] Heat stress affects to the size and phenotype of the rat soleus regenerating fibers (2012)
  • Author(s): Yasuharu Oishi
  • Organizer: 15th International Biochemistry of Exercise Congress (IBEC)
  • Place of Presentation: The Swedish School of Sport and Health Sciences, Stockholm, Sweden.