| Project/Area Number |
24500798
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHNO Hideki 杏林大学, 医学部, 教授 (00133819)
KIZAKI Takako 杏林大学, 医学部, 教授 (00322446)
OGASAWARA Junetsu 杏林大学, 医学部, 助教 (20415110)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脂肪組織 / 運動トレーニング / リモデリング / 細胞外マトリックス / デルマトポンチン / 組織性メタロプロテアーゼ阻害因子1 |
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| Outline of Final Research Achievements |
Recently, several studies have shown that white adipose tissue (WAT) fibrosis is enhanced in obese subjects. In the present study, we examined effects of exercise training (TR) on high fat diet (HFD)-induced expression changes of extracellular matrix (ECM) factors, such as dermatopontin (Dpt), in WAT, and attempted identification of roles of Dpt in WAT. The mRNA levels of Dpt, TGF-β, and TIMP1 in WAT of HFD-induced obese mice were significantly higher than those in WAT of control mice, and TR significantly attenuated the expression of these genes. The production of TIMP1 in 3T3-L1 cells were upregulated by treatment with TGF-β although Dpt did not affect. Moreover, treatment of 3T3-L1 cells with TIMP1 resulted in attenuation of insulin-induced glucose uptake. These results suggest that TR attenuates HFD-induced increases in ECM factors in WAT. Moreover, TR may improve insulin sensitivity by inhibiting accumulation of ECM which is associate with reduction of glucose uptake in WAT.
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