Study of the bone metabolism in diabetes and of improvement by the exercise using newly-introduced diabetes model

• Project/Area Number: 24500848
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Kanazawa University
• Principal Investigator: KITAMURA Kei-ichiro 金沢大学, 保健学系, 教授 (80283117)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Nobuo 金沢大学, 環日本海域環境研究センター, 准教授 (60242476) ; KOGAYA Yasutoku 朝日大学, 歯学部, 准教授 (30076046)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 糖尿病 / 骨代謝 / 糖尿病状態キンギョモデル / アロキサン / 再生ウロコ / 高血糖状態キンギョモデル

Outline of Final Research Achievements

We investigated the cause of the increased fracture rate in diabetes patients through analyses of bone metabolism and the properties of bone matrix protein by using goldfish scales as a bone model of hyperglycemia that is induced by an alloxan injection. The results showed that the marker enzyme activities in the scale osteoblasts and osteoclasts did not significantly differ between hyperglycemic and euglycemic goldfish. This suggests that hyperglycemia has minimal effect on bone metabolism. Moreover, we found that the formation of non-enzymatic cross-linked collagen, which has more than 3 times the molecular weight of a single fiber, was significantly increased in the scale-bone matrix of the hyperglycemic goldfish.
Our results suggest that the increased fracture rate in diabetes patients is mostly due to the loss of flexibility of the collagen fibers, which is mediated by the excessive formation of non-enzymatic cross-linked collagen in the bone matrix.

Research Products

• [Journal Article] Monohydroxylated polycyclic aromatic hydrocarbon influences spicule formation in the early development of sea urchins (Hemicentrotus pulcherrimus). (2015)
  • Author(s): Suzuki, N., Ogiso, S., Yachiguchi, K., Kawabe, K., Makino, F., Toriba, A., Kiyomoto, M., Sekiguchi, T., Tabuchi, Y., Kondo, T., Kitamura, K., Hong, C., Srivastav, A.K., Oshima, Y., Hattori, A., Hayakawa, K.
  • Journal Title: Comp Biochem Physiol C Toxicol Pharmacol. Volume: 171 Pages: 55-60
  • DOI: 10.1016/j.cbpc.2015.02.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of an improved method for quantitative analysis of skin blotting: Increasing reliability and applicability for skin assessment. (2015)
  • Author(s): Ogai K, Matsumoto M, Minematsu H, Kitamura K, Kobayashi M, Sugama J and Sanada H
  • Journal Title: International Journal of Cosmetic Science Volume: - Issue: 4 Pages: 425-432
  • DOI: 10.1111/ics.12217
  • NAID: 120005704522
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone (2014)
  • Author(s): Yachiguchi K, Sekiguchi T, Nakano M, Hattori A, Yamamoto M, Kitamura K, Maeda M, Tabuchi Y, Kondo T, Kamauchi H, Nakabayashi H, Srivastav AK, Hayakawa K, Sakamoto T, Suzuki N
  • Journal Title: Zoolog Sci Volume: 31 Issue: 5 Pages: 330-337
  • DOI: 10.2108/zs130265
  • NAID: 120005463340
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of a novel pulse wave velocity measurement system: Using dual piezoelectric elements. (2014)
  • Author(s): Kitamura, K., Takeuchi, R., Ogai, K., Xin, Z., Chen, W., Nemoto, T.
  • Journal Title: Med Eng Phys. Volume: 36 Issue: 7 Pages: 927-932
  • DOI: 10.1016/j.medengphy.2014.02.024
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of inorganic mercury and methylmercury on osteoclasts and osteoblasts in the scales of the marine teleost as a model system of bone. (2014)
  • Author(s): Yachiguchi, K., Sekiguchi, T., Nakano, M., Hattori, A., Yamamoto, M., Kitamura, K., Maeda, M., Tabuchi, Y., Kondo, T., Kamauchi, H., Nakabayashi, H., Srivastav, A.K., Hayakawa, K., Sakamoto, T. and Suzuki, N.
  • Journal Title: Zool. Sci. Volume: -
  • NAID: 120005463340
  • Peer Reviewed
• [Journal Article] Development of a novel pulse wave velocity measurement system: Using dual piezoelectric elements. (2014)
  • Author(s): Kitamura, K., Takeuchi, R., Ogai, K., Xin, Z., Chen, W., Nemoto, T.
  • Journal Title: Medical Engineering and Physics Volume: -
  • Peer Reviewed
• [Journal Article] Zebrafish scales respond differently to in vitro dynamic and static acceleration: analysis of interaction between osteoblasts and osteoclasts. (2013)
  • Author(s): Kitamura, K., Takahira, K., Inari, M., Satoh, Y., Hayakawa, K., Tabuchi, Y., Ohgai, K., Nishiuchi, T., Kondo, T., Mikuni-Takagi, Y., Chen, X., Hattori, A. and Suzuki, N.
  • Journal Title: Comp Biochem Physiol A Mol Integr Physiol. Volume: 166 Pages: 74-80
  • Peer Reviewed
• [Journal Article] Genes responsive to low-intensity pulsed ultrasound in MC3T3E1 preosteoblast cells. (2013)
  • Author(s): Tabuchi, Y., Sugahara, Y., Ikegame, M., Suzuki, N., Kitamura, K. and Kond, T
  • Journal Title: Int. J. Mol. Sci. Volume: 14 Pages: 22721-22740
  • Peer Reviewed
• [Journal Article] Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales (2013)
  • Author(s): Yano S, et al. (14番目)
  • Journal Title: Zoological Science Volume: 30 Issue: 3 Pages: 217-223
  • DOI: 10.2108/zsj.30.217
  • Peer Reviewed
• [Journal Article] Favorable and unfavorable amino acid residues in water-soluble and transmembrane proteins. (2013)
  • Author(s): Nakashima, H., Yoshihara, A. and Kitamura, K.
  • Journal Title: J. Biomed. Sci. Eng. Volume: 6 Issue: 01 Pages: 36-44
  • DOI: 10.4236/jbise.2013.61006
  • Peer Reviewed
• [Journal Article] Prostaglandin E2 increases both osteoblastic and osteoclastic activities in the scales of goldfish and participates in the calcium metabolism in goldfish (2012)
  • Author(s): Omori, K., Wada, S., Maruyama, Y., Hattori, A., Kitamura, K., Sato, Y., Nara, M., Funahashi, H., Yachiguchi, K., Hayakawa, K., Endo, M., Kusakari, R., Yano, S., Srivastav, A.K., Kusui, T., Ejiri, S., Chen, W., Tabuchi, Y., Furusawa, Y., Kondo, T., Sasayama, Y. Nishiuchi, T., Nakano, M., Sakamoto, T. and Suzuki, N.
  • Journal Title: Zool. Sci Volume: 29 Issue: 8 Pages: 499-504
  • DOI: 10.2108/zsj.29.499
  • Peer Reviewed
• [Journal Article] Determination of Calcium Sensing Receptor in the Scales of Goldfish and Induction of Its mRNA Expression by Acceleration Loading (2012)
  • Author(s): Kakikawa, M., Yamamoto, T., Chowdhury, V.S., Satoh, Y., Kitamura, K., Sekiguchi, T., et al.
  • Journal Title: Biological Sciences in Space Volume: 26 Issue: 0 Pages: 26-31
  • DOI: 10.2187/bss.26.26
  • NAID: 130004450623
  • ISSN: 0914-9201, 1349-967X
  • Peer Reviewed
• [Journal Article] Osteoblasts and Osteoclasts in Regenerating Goldfish Scales Respond to Mechanical Loading: Analysis of Osteoblastic and Osteoclastic Marker mRNA Expression (2012)
  • Author(s): Kitamura, K., Satoh, Y., Inari, M., Takahira, K., Okesaku, W., Endo, M., Yano, S., Yamamoto, T., Kaminishi, A., Akatsuka, R., Hattori, A. and Suzuki, N.
  • Journal Title: Biological Sciences in Space Volume: 26 Issue: 0 Pages: 42-46
  • DOI: 10.2187/bss.26.42
  • NAID: 130004845129
  • ISSN: 0914-9201, 1349-967X
  • Peer Reviewed
• [Journal Article] Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish. (2012)
  • Author(s): Yachiguchi, K., Matsumoto, N., Haga, Y., Suzuki, M., Matsumura, C., Tsurukawa, M., Okuno, T., Nakano, T., Kawabe, K., Kitamura, K., et al.
  • Journal Title: Env. Sci. Poll. Res. Volume: 12 Issue: 10 Pages: 1347-1355
  • DOI: 10.1007/s11356-012-1347-5
  • NAID: 120005253213
  • Peer Reviewed / Open Access
• [Presentation] 骨にいい運動とは？ (2014)
  • Author(s): 北村敬一郎、根本 鉄、鈴木信雄、矢野幸子、田渕 圭章、服部淳彦.
  • Organizer: 日本動物学会中部支部大会
  • Place of Presentation: コンセールのと (石川県鳳珠郡能登町字宇出津ト字 29 番地)
  • Year and Date: 2014-11-22 – 2014-11-24
  • Invited
• [Presentation] 高血糖状態におけるウロココラーゲン繊維への糖化と異常架橋の解析. (2014)
  • Author(s): 桶作若菜、安藤 忠、南 敬生、山岸貴文、服部淳彦、鈴木信雄、北村敬一郎
  • Organizer: 日本動物学会中部支部大会
  • Place of Presentation: コンセールのと (石川県鳳珠郡能登町字宇出津ト字 29 番地 )
  • Year and Date: 2014-11-22 – 2014-11-24
• [Presentation] Characterization of Ultradian and Circadian Rhythms of Core Temperature Based on Wavelet Analysis. (2014)
  • Author(s): Huang M, Tamura T, Chen W, Kitamura K, Nemoto T and Kanaya S.
  • Organizer: 36th Annual International IEEE EMBS Conference 2014
  • Place of Presentation: Chicago, Illinois, USA.
  • Year and Date: 2014-08-26 – 2014-08-30
• [Presentation] Response of osteoclasts and receptor activator of nuclear factor kappa-B ligand expression in the regenerating scales of goldfish under microgravity. (2014)
  • Author(s): Ikegame M, Hattori A, Tabuchi Y, Kitamura K, Yamamoto T, Nakano M, Yanao S, Yamamoto T, Suzuki N.
  • Organizer: International Symposium on Mechanobilogy (ISMB) 2014
  • Place of Presentation: Junko Fukutake Hall, Okayama, Japan
  • Year and Date: 2014-05-20 – 2014-05-23
  • Invited
• [Presentation] 新規メラトニン誘導体の骨代謝に対する作用：魚類及び哺乳類におけるin vivoの解析 (2014)
  • Author(s): 鈴木信雄・関口俊男・帖地 藍・山本 樹・上西篤志・関あずさ・高垣裕子・池亀美華・田渕圭章・近藤 隆・北村敬一郎・清水宣明・矢野幸子・染井正徳・服部淳彦
  • Organizer: 日本動物学会中部支部大会
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター (愛知県)
• [Presentation] キンギョを用いた糖尿病様モデルのインスリン産生能の解析. (2013)
  • Author(s): 北村敬一郎、安藤 忠、桶作若菜、遠藤雅人、服部淳彦、鈴木信雄
  • Organizer: 第3８回日本比較内分泌学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県）
• [Presentation] IDENTIFICATION OF GENES RESPONSIVE TO LOW-INTENSITY PULSED ULTRASOUND IN MOUSE PREOSTEOBLAST CELLS. (2013)
  • Author(s): Tabuchi Y, Sugahara Y, Ikegame M, Suzuki N, Kitamura K, Kondo T.
  • Organizer: International Conference on Biomedical Ultrasound 2013
  • Place of Presentation: Taipei International Convention Center (Taipei)
• [Presentation] Improvement of the dual-heat-flux method for deep body temperature measurement based on a finite element model. (2013)
  • Author(s): Huang M, Chen W, Kitamura K, Nemoto T, Tamura T.
  • Organizer: 35th Annual International Conference of the IEEE
  • Place of Presentation: Osaka International Conference Center (Osaka)
• [Presentation] 魚類の骨代謝に対する多環芳香族炭化水素類の影響評価：再生ウロコを用いた解析. (2012)
  • Author(s): 鈴木信雄, 川部季美, 中野 淳, 北村敬一郎, 他
  • Organizer: 日本動物学会 中部支部大会
  • Place of Presentation: 松本市浅間温泉みやま荘 (長野県)
• [Presentation] 糖尿病状態キンギョのモデルの作製. (2012)
  • Author(s): 北村敬一郎、安藤 忠、室石洋子、河村常作、小田惠夫、服部淳彦、鈴木信雄
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井大学文京キャンパス （福井県）
• [Remarks] 研究者情報
  • URL: http://ridb.kanazawa-u.ac.jp/public/detail.php?id=2619
• [Remarks] 金沢大学研究者情報
  • URL: http://ridb.kanazawa-u.ac.jp/public/detail.php?id=2619