An investigation of endocrine factors from cultured skeletal muscle cells and the effect of cell pressurization
| Project/Area Number |
24500864
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
IIZUKA Kenji 北海道医療大学, 薬学部, 教授 (10344467)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAFUJI Masahiko 北海道医療大学, 薬学部, 教授 (20142987)
MACHIDA Takuji 北海道医療大学, 薬学部, 講師 (90433424)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 骨格筋細胞 / ミオカイン / 培養骨格筋細胞 / マーカー / 増殖因子 / 骨格筋 / 擬似的運動負荷 / エネルギー代謝 |
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| Outline of Final Research Achievements |
Human skeletal muscle cells (Huskmcs) were cultured and pressurized to spuriously reproduce an intra-muscular pressure elevation during exercise. Investigations of mRNA expression in pressurized Huskmcs resulted in an increase in several secreted endocrine factors known as myokines, and protein levels in culture medium were also increased compared with non-pressurized cells. An administration of culture medium, collected by pressurized Huskmcs, to human adipocytes resulted in an increase in more than two times of up-regulation in cytokine release into culture medium. Huskmcs may play as a secretory organ during exercise and those released factors may have effect on adipocytes as a myokine.
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Report
(4 results)
Research Products
(5 results)
