| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Outline of Final Research Achievements |
In our previous study, we found experimental results which contradict with the theory of aging that reactive oxygen species (ROS) generation increases with energy metabolism. In this study, we developed the combined apparatus, real-time bioradiography and oxygen micro-sensor in tissues slices. The ROS generation and tissue oxygen concentration were measured during hypoxia-reoxygenation using this system. ROS generation was enhanced during reoxygenation after the low oxygen supply caused hypoxic treatment. Similarly, enhanced energy demand in tissue caused hypoxia enhanced the ROS generation during reoxygenation. Low tissue oxygen concentration was confirmed in both of hypoxia models. The hypoxic condition would be cause of “hyper-reduction”, therefore the as a future study, the relationship between ROS generation and redox state in tissue is required.
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