Elucidation of the effect of polyphenols on the inhibition of intestinal cholesterol transport and for their clinical applications
| Project/Area Number |
24500969
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOBAYASHI Shoko 東京大学, 農学生命科学研究科, 准教授 (90348144)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIMARU Yoshiro 東京大学, 大学院農学生命科学研究科, 特任准教授 (10451840)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | コレステロールトランスポーター / ルテオリン / ケルセチン / コレステロール / トランスポーター / ポリフェノール / フラボノイド / 腸管吸収抑制 / NPC1L1 / 腸管 |
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| Outline of Final Research Achievements |
Niemann-pick C1-Like 1 (NPC1L1) mediates cholesterol absorption, and ezetimibe is a potent NPC1L1 inhibitor applicable for medication of hypercholesterolemia. Epidemiological studies demonstrated that consumption of polyphenols correlates with a decreased risk for atherosclerosis, and we hypothesized that polyphenols inhibit intestinal transport of cholesterol. We elucidated the kinetic parameters of intestinal cholesterol absorption using Caco-2 cells and screened 34 polyphenols. Luteolin and quercetin inhibited cholesterol absorption by Caco-2 cells and human embryonic kidney 293T cells expressing NPC1L1. Serum cholesterol levels significantly decreased more in rats fed both cholesterol and luteolin (or quercetin), than in those observed in the cholesterol feeding group. These results suggest that luteolin and quercetin reduce high blood cholesterol levels by specifically inhibiting intestinal cholesterol absorption mediated by NPC1L1.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Identification of a New IgE-Binding Epitope of Peanut Oleosin That Cross-Reacts with Buckwheat2012
Author(s)
Kobayashi, S., Katsuyama, S., Wagatsuma, T., Okada, S., and Tanabe, S
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 76
Issue: 6
Pages: 1182-1188
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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