Mechanism of anti-inflammatory property of dietary fiber through glucan receptor on small intestinal epitherial cells

• Project/Area Number: 24500974
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kobe University
• Principal Investigator: MIZUNO Masashi 神戸大学, (連合)農学研究科(研究院), 教授 (00212233)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: Lentinan / Dectin-1 / 多糖類 / 抗炎症作用 / レンチナン / 炎症生腸疾患 / βグルカン / 腫瘍壊死因子受容体 / 小腸上皮細胞 / 樹状細胞 / 炎症性腸疾患 / インターロイキン8

Outline of Final Research Achievements

In this study, the mechanism of anti-inflammation by lentinan was investigated. Lentinan was orally administered to Dectin-1 knockout (Dectin-1 KO) mice which were treated by DSS to induce colitis. Oral administration of lentinan (100 μg/mouse/day) did not show any significant improvement in DSS-induced Dectin-1 KO mice. Moreover, TNFR1 mRNA expression in intestinal epithelial cells was decreased by lentinan administration to wild type mice, but not Dectin-1 KO mice. These results revealed that lentinan may exert its anti-inflammatory activities through the beta-glucan receptor Dectin-1 with the decrease of TNFR1 in intestinal epithelial cells.

Research Products

• [Journal Article] Lentinan degradation in the lentinula edodes fruiting body during postharvest preservation is reduced by downregulation of the exo-β-1,3-glucanase EXG2 (2014)
  • Author(s): Konno, N., Nakade, K., Nishitani, Y., Mizuno, M. and Sakamoto, Y.
  • Journal Title: J. Agric. Food Chem. Volume: 62 Issue: 32 Pages: 8153-8157
  • DOI: 10.1021/jf501578w
  • Peer Reviewed
• [Journal Article] Macrophage activation-mediated hydrogen peroxide generation by the royal sun medicinal mushroom Agaricus brasiliensis (higher Basidiomycetes) (2013)
  • Author(s): Mizuno Masashi and Nishitani Yosuke
  • Journal Title: International Journal of Medicinal Mushrooms Volume: 15 Pages: 365-371
  • Peer Reviewed
• [Journal Article] Immunomodulating compounds in Basidiomycetes (2013)
  • Author(s): Mizuno Masashi and Nishitani Yosuke
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 52 Pages: 202-207
  • NAID: 130004879333
  • Peer Reviewed
• [Journal Article] Intestinal anti-inflammatory activity of lentinan: influence on IL-8 and TNFR1 expression in intestinal epithelial cells. (2013)
  • Author(s): Nishitani Y, Zhang L, Yoshida M, Azuma T, Kanazawa K, Hashimoto T, Mizuno M.
  • Journal Title: PlosOne Volume: 8(4)
  • NAID: 120005602547
  • Peer Reviewed
• [Presentation] 機能性多糖レンチナンは小腸上皮細胞を介して免疫応答を制御する (2015)
  • Author(s): 大浦圭吾、橋本堂史、水野雅史
  • Organizer: 日本農芸化学会関西支部第488回講演会
  • Place of Presentation: 京都大学楽友会館
  • Year and Date: 2015-01-31
• [Presentation] 食品由来多糖によるナイーブＴ細胞分化の制御 (2014)
  • Author(s): 大浦圭吾、橋本堂史、水野雅史
  • Organizer: 日本応用糖質科学会平成26年度大会
  • Place of Presentation: 朱鷺メッセ新潟コンベンションセンター
  • Year and Date: 2014-09-24
• [Presentation] 食品に含まれる機能成分による腸管免疫制御機構 (2014)
  • Author(s): 水野雅史
  • Organizer: 無し
  • Place of Presentation: 大阪薬業年金会館
  • Year and Date: 2014-09-03
  • Invited
• [Presentation] シイタケは腸を健全に保ちます (2014)
  • Author(s): 水野雅史
  • Organizer: 無し
  • Place of Presentation: 吉備国大大学
  • Year and Date: 2014-08-06
  • Invited
• [Presentation] レンチナンによる腸炎抑制を引き起こす小腸上皮細胞上受容体について (2013)
  • Author(s): 雉子谷百合江、西谷洋輔、橋本堂史、水野雅史
  • Organizer: 日本応用糖質科学会平成25年度大会（第62回）
  • Place of Presentation: 鹿児島大学
• [Presentation] Lentinan, β-1,3;1,6-glucan, exerts intestinal anti-inflammatory activity through Dectin-1 (2013)
  • Author(s): Mizuno, M., Kijitani, Y. and Hashimoto, T.
  • Organizer: Eurpoean Polysaccharide Network of Excellence
  • Place of Presentation: Nice Acropolis Convention and Exhibition Center (France)
• [Presentation] Dectin-1 is responsible for intestinal anti-inflammatory activity of Lentinula edodes extract lentinan (2013)
  • Author(s): Kijitani, Y. and Mizuno, M.
  • Organizer: Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: 幕張メッセ
• [Presentation] レンチナンは腸管上皮細胞のTNFR1の発現制御によって腸管炎症を抑制する (2012)
  • Author(s): 西谷洋輔、水野雅史
  • Organizer: 腸内細菌学会
  • Place of Presentation: 神戸
• [Presentation] Endocytosis of tumor necrosis factor receptor 1 plays a key role in anti-inflammatory by lentinan in intestinal epithelial cells (2012)
  • Author(s): Masashi Mizuno, Ling Zhang and Yosuke Nishitani
  • Organizer: The 3rd Conference for the African Society for Edible and Medicinal Mushrooms
  • Place of Presentation: Windhoek
  • Invited