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(-)-Epigallocatechin-3-gallate increases the number of neural stem cells around the damaged area after rat traumatic brain injury

Research Project

Project/Area Number 24501009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionKinki University

Principal Investigator

SATOU Takao  近畿大学, 医学部附属病院, 教授 (70162443)

Co-Investigator(Kenkyū-buntansha) ITOH Tatsuki  近畿大学, 農学部, 教授 (40330245)
INOUE Takao  近畿大学, 医学部, 助教 (00441006)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords脳外傷 / 神経再生 / カテキン / 緑茶飲料 / 神経幹細胞 / 緑茶 / 神経再生促進 / 酸化ストレス / 神経保護作用
Outline of Final Research Achievements

A major component of green tea is (-)-epigallocatechin gallate (EGCG), which has strong antioxidant properties. we investigated the effect of EGCG on neural stem cell (NSC) proliferation following traumatic brain injury (TBI). Male Wistar rats that had access to normal drinking water, or water containing 0.1% (w/v) EGCG, ad libitum. Immunohistochemistry revealed that the number of nestin-positive cells after TBI in the EGCG group increased significantly. The number of single-stranded DNA (ssDNA)-positive cells after TBI significantly decreased in the EGCG group. Almost all ssDNA-positive cells in the water group co-localized with NeuN and nestin-staining. Spheres could only be isolated in the water group at 3 days.In the EGCG group, spheres could be isolated at all days following TBI. Spheres differentiated into neurons and glia. These results indicate that EGCG inhibits degradation of NSCs, which have the potential to differentiate into neurons and glia following TBI.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] By inhibiting Src, verapamil and dasatinib overcome multidrug resistance via increased expression of Bim and decreased expressions of MDR1 and survivin in human multidrug-resistant myeloma cells.2014

    • Author(s)
      Tsubaki M, Komai M, Itoh T, Imano M, Sakamoto K, Shimaoka H, Takeda T, Ogawa N, Mashimo K, Fujiwara D, Mukai J, Sakaguchi K, Satou T, Nishida S
    • Journal Title

      Leuk Res

      Volume: 38 Issue: 1 Pages: 120-130

    • DOI

      10.1016/j.leukres.2013.10.017

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cutaneous lymphangioma circumscriptum with marked blood presence: histopathologic evaluation of the endothelial cells2014

    • Author(s)
      Oiso N, Itoh T, Miyake M, Satou T, Kawada A
    • Journal Title

      Eur J Dermatol

      Volume: 24 Issue: 1 Pages: 127-128

    • DOI

      10.1684/ejd.2013.2248

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased apoptotic neuronal cell death and cerebral dysfunction after traumatic brain injury in aged rats2012

    • Author(s)
      Itoh T, Imano M, Nishida S, Tsubaki M, Hashimoto S, Ito A, Satou T
    • Journal Title

      Brain Struct Func

      Volume: (in press) Issue: 1 Pages: 209-220

    • DOI

      10.1007/s00429-012-0394-5

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] (-)-Epigallocatechin-3-gallate increases the number of neural stem cells around the damaged area after rat traumatic brain injury2012

    • Author(s)
      Itoh T, Imano M, Nishida S, Tsubaki M, Hashimoto S, Ito A, Satou T
    • Journal Title

      J Neural Transm

      Volume: (in press) Issue: 8 Pages: 877-890

    • DOI

      10.1007/s00702-011-0764-9

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] エピガロカテキンガレートの実験的脳外傷後に出現する神経幹細胞への影響2014

    • Author(s)
      佐藤隆夫、伊藤龍生
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脳外傷前後の緑茶飲料による神経保護作用の検討2014

    • Author(s)
      伊藤龍生、佐藤隆夫
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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