| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Chromosomal translocations are mediated by illegitimate joining between distal parts of the different chromosomes. But the mechanism of proximity of these ends remains unknown which should have been repaired precisely. In this study, I used a palindromic sequence that induces frequent (1/20,000) de novo translocation in sperm from healthy men, and analyzed the interaction of these specific sequences that form DNA secondary structures in vivo. I found that the secondary structures were cleaved by two endogenous DNA repair enzymes, that the other DNA secondary structure also influences the chromosome metabolisms. Because of the difficulty of the DNA secondary structure, the chromosome conformation analysis remains a future subject.
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