Molecular mechanisms of integrin endocytosis

• Project/Area Number: 24501316
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Kumamoto University
• Principal Investigator: UMEDA Kazuaki 熊本大学, 大学院生命科学研究部, 講師 (80444876)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: インテグリン / エンドサイトーシス

Outline of Final Research Achievements

Integrin heterodimers, consisting of alpha- and beta- chains, are cell surface receptors involved in adhesion between cells and extracellular matrix. They play important roles in various cellular events such as cell cytokinesis, differentiation and migration. Therefore it is important to elucidate the molecular mechanisms by which the amount of integrins on the cell surface are regulated. However the mechanisms remains unknown precisely. We identified that integrin alpha5beta1 are ubiquitinated by Nedd4L, an E3 ubiquitin ligase, during endocytosis.