| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Outline of Final Research Achievements |
Our immunohistochemical analyses and genetic lineage study showed that the medulloblastoma (MB) were composed of the MB cells of varied differentiation status, and that the cancer stem cells took the bipolar cell morphology and often differentiated into neurons with multiple dendrites like normal immature cerebellar granule neurons. We found that in MB, the expression patterns of miRNA-Xs that we have identified as the gatekeeper in MB development, were well correlated with the differentiation state of MB cells. Mis-expression of miRNA-X induced the differentiation of MB stem cell lines (in vitro), and the simultaneous inhibition of miRNA-Xs with Ptc1 cKO in granule cell progenitor cells induced the cancer stem cell-dominant MB (in vivo).
These results indicate that miRNA-Xs must be involved in making the hierarchy of MB cells, promising their application to eradicate the cancer stem cells for MB cure.
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