Quantitative anlysis on antigenic peptides from the tumor antigen and its application to a biomarker for cancer immunotherapy
| Project/Area Number |
24501334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor immunology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
HOMMA SADAMU 東京慈恵会医科大学, 医学部, 教授 (50192323)
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| Co-Investigator(Kenkyū-buntansha) |
IWAMOTO Takeo 東京慈恵会医科大学, 分子細胞生物学研究部, 准教授 (90568891)
KOIDO Shigeo 東京慈恵会医科大学, 消化器・肝臓内科, 准教授 (70266617)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | がんワクチン / WT1 / 質量解析 / 膵癌 / LC/MS/MS / Mass imaging / 抗原性ペプチド / 腫瘍抗原 / 腫瘍組織標本 / がん免疫療法 |
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| Outline of Final Research Achievements |
Presentation of antigenic peptide of the tumor antigen on the HLA class I molecule is an essential factor for effective antigen-specific cancer vaccine. Using mass spectrometric analyses, identification and quantification of the HLA-A24 restricted antigenic peptide of Wilms' Tumor-1 (WT1) were tried in human pancreatic cancer zenograft tissue. Antigenic peptides binding to HLA class I molecules were obtained from the tumor cells by acid-elusion method and analyzed for detection of WT1 antigenic peptide using LC/MS/MS. WT1 antigenic peptide (CMTWNQMNL) was identified as a single ion peak and a standard curve for quantification could be generated. Furthermore, spots of WT1 antigenic peptide were observed in the tumor tissue by mass imaging analysis. Presentation of antigenic peptide of the tumor antigen on MHC class I molecules in tumor tissue could be defined by mass spectrometric examination.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Prognostic Markers for Patient Outcome Following Vaccination with Multiple MHC Class I/II-restricted WT1 Peptide-pulsed Dendritic Cells Plus Chemotherapy for Pancreatic Cancer.2015
Author(s)
2.Takakura K, Koido S, Kan S, Yoshida K, Mori M, Hirano Y, Ito Z, Kobayashi H, Takami S, Matsumoto Y, Kajihara M, Misawa T, Okamoto M, Sugiyama H, Homma S, Ohkusa T, Tajiri H.
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Journal Title
Anticancer Research
Volume: 35
Pages: 555-562
Related Report
Peer Reviewed
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[Journal Article] Immunochemoradiotherapy for patients with oral squamous cell carcinoma: augmentation of OK-432-induced helper T cell 1 response by 5-FU and X-ray irradiation2013
Author(s)
Tano T, Okamoto M, Kan S, Bando T, Goda H, Nakashiro K, Shimodaira S, Koido S, Homma S, Fujita T, Sato M, Yamashita N, Hamakawa H, Kawakami Y
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Journal Title
Neoplasia
Volume: 15
Pages: 805-814
Related Report
Peer Reviewed
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[Journal Article] Immunotherapy for colorectal cancer2013
Author(s)
Koido S, Ohkusa T, Homma S, Namiki Y, Takakura K, Saito K, Ito Z, Kobayashi H, Kajihara M, Uchiyama K, Arihiro S, Arakawa H, Okamoto M, Gong J, Tajiri H
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Journal Title
World Journal of Gastroenterology
Volume: 19
Issue: 46
Pages: 8531-8542
DOI
Related Report
Peer Reviewed
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[Presentation] 進行膵癌に対するWT1ペプチドワクチンとゲムシタビンによる集学的治療法の選択基準2013
Author(s)
小井戸 薫雄, 西田 純幸, 本間 定, 込田 英夫, 高原 映崇, 高倉 一樹, 内山 幹, 今津 博雄, 荒川 廣志, 大草 敏史, 田尻 久雄
Organizer
第５５回日本消化器病学会大会
Place of Presentation
東京
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