Developments of A New Antibody Library and Antibody Chips

• Project/Area Number: 24501343
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: Keio University
• Principal Investigator: TAKAYANAGI Atsushi 慶應義塾大学, 医学部, 講師 (80245464)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: EGFレセプター / 抗体 / ライブラリー / 抗体ライブラリー / IGFレセプター / アポトーシス / リン酸化

Outline of Final Research Achievements

(1)We have analyzed effects on tumor cells of anti-EGF receptor antibodies recognized unique epitopes, which were obtained form our original antibody library. The antibodies induced apoptosis to EGFR-overproduced tumor cells with wild type and several tumorigenic K-RAS mutations. The antibodies inhibited EGF-induced EGFR phosphorylation and Akt phosphorylation.
(2)For we have constructed an Fab antibody-displying phage library with 10^18 repertory which are based on new concept for library-screening system.

Research Products

• [Journal Article] Therapeutic Potential of Transgenic Mesenchymal Stem Cells Engineered to Mediate Anti-HMGB1 Activity: Targeting of Colon Cancer (2014)
  • Author(s): Kikuchi, H., Yagi, H., Hasegawa, H., Ishii, Y., Okabayashi, K., Tsuruta, M., Hoshino, G., Takayanagi, A., Kitagawa, Y.
  • Journal Title: Journal of Surgical Research Volume: S0022-4804 Issue: 1 Pages: 00188-00197
  • DOI: 10.1016/j.jss.2014.02.047
  • Peer Reviewed
• [Journal Article] Recombinant Human IgG Antibodies Recognizing Distinct Extracellular Domains of EGF Receptor Exhibit Different Degrees of Growth Inhibitory Effects On Human A431 Cancer Cells. (2013)
  • Author(s): Chang, C., Takayanagi, A., Yoshida, T., Shimizu, N.
  • Journal Title: Experimental Cell Research Volume: 319 Issue: 8 Pages: 1146-1155
  • DOI: 10.1016/j.yexcr.2013.03.002
  • Peer Reviewed
• [Presentation] A New Periplasmic Chaperon Vector for Improvement of Phage-Displayed Antibody Library (2012)
  • Author(s): Atsushi Takayanagi, Tetsuhiko Yoshida, Nobuyoshi Shimizu
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Patent(Industrial Property Rights)] Antibody and Use Thereof (2012)
  • Inventor(s): 清水信義、高柳淳、吉田徹彦
  • Industrial Property Rights Holder: 慶應義塾大学、東亞合成株式会社
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-12-04
  • Overseas