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Developments of A New Antibody Library and Antibody Chips

Research Project

Project/Area Number 24501343
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor diagnosis
Research InstitutionKeio University

Principal Investigator

TAKAYANAGI Atsushi  慶應義塾大学, 医学部, 講師 (80245464)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsEGFレセプター / 抗体 / ライブラリー / 抗体ライブラリー / IGFレセプター / アポトーシス / リン酸化
Outline of Final Research Achievements

(1)We have analyzed effects on tumor cells of anti-EGF receptor antibodies recognized unique epitopes, which were obtained form our original antibody library. The antibodies induced apoptosis to EGFR-overproduced tumor cells with wild type and several tumorigenic K-RAS mutations. The antibodies inhibited EGF-induced EGFR phosphorylation and Akt phosphorylation.
(2)For we have constructed an Fab antibody-displying phage library with 10^18 repertory which are based on new concept for library-screening system.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Therapeutic Potential of Transgenic Mesenchymal Stem Cells Engineered to Mediate Anti-HMGB1 Activity: Targeting of Colon Cancer2014

    • Author(s)
      Kikuchi, H., Yagi, H., Hasegawa, H., Ishii, Y., Okabayashi, K., Tsuruta, M., Hoshino, G., Takayanagi, A., Kitagawa, Y.
    • Journal Title

      Journal of Surgical Research

      Volume: S0022-4804 Issue: 1 Pages: 00188-00197

    • DOI

      10.1016/j.jss.2014.02.047

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Recombinant Human IgG Antibodies Recognizing Distinct Extracellular Domains of EGF Receptor Exhibit Different Degrees of Growth Inhibitory Effects On Human A431 Cancer Cells.2013

    • Author(s)
      Chang, C., Takayanagi, A., Yoshida, T., Shimizu, N.
    • Journal Title

      Experimental Cell Research

      Volume: 319 Issue: 8 Pages: 1146-1155

    • DOI

      10.1016/j.yexcr.2013.03.002

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] A New Periplasmic Chaperon Vector for Improvement of Phage-Displayed Antibody Library2012

    • Author(s)
      Atsushi Takayanagi, Tetsuhiko Yoshida, Nobuyoshi Shimizu
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] Antibody and Use Thereof2012

    • Inventor(s)
      清水信義、高柳淳、吉田徹彦
    • Industrial Property Rights Holder
      慶應義塾大学、東亞合成株式会社
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-12-04
    • Related Report
      2012 Research-status Report
    • Overseas

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Published: 2013-05-31   Modified: 2019-07-29  

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