| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Detection of HPV in oropharyngeal squamous cell carcinomas (OCs) is important because HPV-positive OCs are more sensitive to chemo-radiotherapy. We analyzed the HPV status in 119 OC cases (F:M=18:101), using formalin-fixed, paraffin-embedded materials, and 13 type-specific probes of Clinichip HPV (Sekisui), nested PCR and in situ hybridization.
HPV DNA was detected in 74 of 119 cases (62%), F:M=15:59, while HPV was negative in 45 of 119 cases (38%), F:M=3:42. Typing analysis revealed that 63, 3, 1, 4, 1 and 2 cases harboring type16, 18, 31, 35, 58 and plural, respectively.
In conclusion, HPV was detected in 62% of Japanese OC cases, of which type 16 was by far the most frequent (85%). Ectopic chromosome around centrosome (ECAC) was detected in 69% of HPV-positive cases but never in HPV-negative cases. The ECAC was thus a distinct marker for the high risk HPV association of OCs.
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