Cancer therapeutic target identification by comprehensive kinase profiling
| Project/Area Number | 24501363 |
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Clinical oncology
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| Research Institution | Wakayama Medical University (2014)
Shizuoka Cancer Center Research Institute (2012-2013) |
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Principal Investigator |
KOH Yasuhiro 和歌山県立医科大学, 医学部, 講師 (80426519)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Nobuyuki 和歌山県立医科大学, 医学部, 教授 (60298966)
YASUI Hirofumi 静岡県立静岡がんセンター, 副院長
NAKAJIMA Takashi 静岡県立静岡がんセンター, 病理診断科, 部長 (20124422)
KONDO Haruhiko 杏林大学, 呼吸器・甲状腺外科, 教授 (60399590)
ENDO Masahiro 静岡県立静岡がんセンター, 画像診断科, 部長
BOKU Narikazu 国立がん研究センター中央病院, 副院長 (50505948)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed(Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost : ¥4,100,000、Indirect Cost : ¥1,230,000)
Fiscal Year 2014 : ¥780,000 (Direct Cost : ¥600,000、Indirect Cost : ¥180,000)
Fiscal Year 2013 : ¥2,470,000 (Direct Cost : ¥1,900,000、Indirect Cost : ¥570,000)
Fiscal Year 2012 : ¥2,080,000 (Direct Cost : ¥1,600,000、Indirect Cost : ¥480,000)
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| Keywords | 分子標的治療 / チロシンキナーゼ / スキルス胃がん / 肺がん |
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| Outline of Final Research Achievements |
In this project, we incorporated peptide microarray system to evaluate the kinase activities of cancer cells and tissues for novel therapeutic targets and predictive biomarker identification.
Kinase activity signature specific to scirrhous-type gastric cancer were successfully identified using non-scirrhous and scirrhous gastric cancer cell lines according to basal kinase activities of tyrosine kinases. Moreover scirrhous gastric cancer cells were sensitive to multikinase inhibotors. Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors, gefitinib, erlotinib and afatinib were compared using EGFR-mutant and –negative cell lines and inhibition profiles turned out to be very different among three inhibitors. In clinical study using tumor and normal tissues from small-cell lung cancer patients, novel therapeutic targets were successfully identified. Src inhibition was suggested to be novel therapeutic strategy for small-cell lung cancer.
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Report
(4results)
Research Products
(30results)
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[Journal Article] Biological significance of 18F-FDG uptake on PET in patients with non-small-cell lung cancer.2014
Author(s)
Kaira K, Serizawa M, Koh Y, Takahashi T, Yamaguchi A, Hanaoka H, Oriuchi N, Endo M, Ohde Y, Nakajima T, Yamamoto N.
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Journal Title
Lung Cancer
Volume : 83
Pages : 197-204
DOI
Related Report
Peer Reviewed
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[Journal Article] Size-based isolation of circulating tumor cells in lung cancer patients using a microcavity array system2013
Author(s)
Masahito Hosokawa, Hirotsugu Kenmotsu, Yasuhiro Koh, Tomoko Yoshino, Takayuki Yoshikawa, Tateaki Naito, Toshiaki Takahashi, Haruyasu Murakami, Yukiko Nakamura, Asuka Tsuya, Takehito Shukuya, Akira Ono, Hiroaki Akamatsu, Reiko Watanabe, Sachiyo Ono, Keita Mori, Hisashige Kanbara, Ken Yamaguchi, Tsuyoshi Tanaka, Tadashi Matsunaga, Nobuyuki Yamamoto
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Journal Title
DOI
Related Report
Peer Reviewed
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[Journal Article] Microcavity array system for size-based enrichment of circulating tumor cells from the blood of patients with small-cell lung cancer2013
Author(s)
Masahito Hosokawa, Takayuki Yoshikawa, Ryo Negishi, Tomoko Yoshino, Yasuhiro Koh, Hirotsugu Kenmotsu, Tateaki Naito, Toshiaki Takahashi, Nobuyuki Yamamoto, Yoshihito Kikuhara, Hisashige Kanbara, Tsuyoshi Tanaka, Ken Yamaguchi. Tadashi Matsunaga
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Journal Title
Analytical Chemistry
Volume : 85
Pages : 5692-5698
DOI
Related Report
Peer Reviewed
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[Journal Article] A microarray-based gene expression analysis to identify diagnostic biomarkers for unknown primary cancer2013
Author(s)
Kurahashi I, Fujita Y, Arao T, Kurata T, Koh Y, Sakai K, Matsumoto K, Tanioka M, Takeda K, Takiguchi Y, Yamamoto N, Tsuya A, Matsubara N, Mukai H, Minami H, Chayahara N, Yamanaka Y, Miwa K, Takahashi S, Takahashi S, Nakagawa K, Nishio K
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DOI
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Peer Reviewed
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