| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
For better understanding of the dynamics of low-molecular weight dissolved organic matter in marine environments, we developed a novel technique to determine oligosaccharides concentrations in seawater. Seawater samples were desalted by solid phase extraction using with a graphite carbon column and evaporated to concentrate oligosaccharides, then analyzed by high performance anion exchange chromatography-pulsed amperometric detection. We determined the concentrations of sucrose, maltose, trehalose and lactulose in the epipelagic layer of the Suruga Bay, and detected other six unknown-oligosaccharides. MPN survey indicated high abundance of prokaryotes in the Suruga Bay that were grown on sucrose, maltose and trehalose. Our findings demonstrated, for the first time, bottom-up controls of prokaryotes by dissolved organic matter on a molecular level in seawater.
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