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Estimation of Organic Carbon Content of the Cyanobacterium by Soft X-ray Microscopy

Research Project

Project/Area Number 24510039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental impact assessment/Environmental policy
Research InstitutionKansai Medical University

Principal Investigator

KUSUMOTO KUNIKO (TAKEMOTO KUNIKO)  関西医科大学, 医学部, 准教授 (80281509)

Co-Investigator(Renkei-kenkyūsha) YAMAMOTO Akitsugu  長浜バイオ大学, バイオサイエンス学部, 教授 (30174775)
IKEGAYA Hisato  兵庫県立大学, 理学部, 客員研究員 (30531579)
Research Collaborator ICHISE Satoshi  滋賀県琵琶湖環境科学研究センター, 環境監視部門, 専門員
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords軟X線顕微鏡 / 微細シアノバクテリア / 細胞外代謝産物(EPS) / Synechococcus sp. / 有機物量評価 / X線マイクロCT / 細胞外代謝産物 / シアノバクテリア / 軟X線顕微鏡 / 粒子性有機炭素 / X線マイクロCT / 炭素量換算 / 粒子性有機炭素(POC)
Outline of Final Research Achievements

Phytoplankton typically produces large amounts of extracellular polysaccharides (EPS), which is mainly composed of macromolecular heteropolysaccharides. As EPS contains large quantities of organic matter, it may be a major contributor to the increased COD in Lake Biwa. To confirm this speculation, a method for the accurate evaluation of the organic carbon (OC) content of phytoplankton by soft X-ray microscopy (XM) was developed and applied to Synechococcus sp. Soft XM is a non-destructive imaging technique that generates images based on differences in X-ray absorption by light elements, such as, carbon, oxygen, and nitrogen. Based on the X-ray absorption coefficients and gray levels of the XM images, the OC content of EPS-covered cells (0.39-0.47 pg/cell) was found to be 2.0-2.4 times larger than that of EPS-removed cells (0.20 pg/cell). These findings suggest that soft XM could be a useful tool for evaluating the OC content of picophytoplankton and EPS without pretreatment steps.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2015 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 3 results) Presentation (13 results) (of which Invited: 1 results)

  • [Journal Article] Estimation of Organic Carbon Content of the Cyanobacterium Synechococcus sp. by Soft X-ray Microscopy2015

    • Author(s)
      H. Ikegaya, S. Suzuki, S. Ichise, S. Furuta, S. Wakabayashi, T. Ohigashi, D. Bamba, H. Namba, H. Kihara, N. Kishimoto, K. Takemoto
    • Journal Title

      Geomicrobiology Journal

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 軟X線顕微鏡による琵琶湖産糸状カビ臭産生藍藻の細胞内微細構造観察2014

    • Author(s)
      竹本邦子、吉村真史、大東琢治、山本章嗣、一瀬諭、難波秀利、木原裕
    • Journal Title

      放射光

      Volume: 27 Pages: 258-266

    • NAID

      40020229000

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 軟X線顕微鏡と蛍光顕微鏡によるシアノバクテリアの観察2014

    • Author(s)
      竹本邦子
    • Journal Title

      応用物理

      Volume: 83 Pages: 293-296

    • NAID

      130007718498

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 軟X線顕微鏡によるバイオイメージング2014

    • Author(s)
      竹本邦子
    • Journal Title

      OplusE

      Volume: 36 Pages: 301-305

    • Related Report
      2013 Research-status Report
  • [Journal Article] Soft x-ray imaging of intracellular granules of filamentous cyanobacterium generating musty smell in Lake Biwa2013

    • Author(s)
      K. Takemoto, G. Mizuta, A. Yamamoto, M. Yoshimura, S. Ichise, H. Namba and H. Kihara
    • Journal Title

      11th International Conference on X-ray Microscopy (XRM2012), Journal of Physics: Conference Series

      Volume: 463 Pages: 12052-12052

    • DOI

      10.1088/1742-6596/463/1/012052

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 琵琶湖産の糸状カビ臭産生藍藻Phormidium tenueの細胞内微細構造観察:軟X線顕微鏡と透過型電子顕微鏡および低真空クライオ走査型電子顕微鏡を用いた比較観察2012

    • Author(s)
      竹本邦子, 山本章嗣, 水田 剛, 一瀬 諭, 吉村真史, 難波秀利, 木原 裕
    • Journal Title

      日本水処理生物学会誌

      Volume: 48 Pages: 157-163

    • NAID

      10031145966

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 結像型軟X線顕微鏡のクライオ機構の改良とCT観察2015

    • Author(s)
      吉村真史,石井達也,村上翔一,竹本邦子,木原裕,難波秀利,太田俊明
    • Organizer
      日本放射光学会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス(滋賀県草津)
    • Year and Date
      2015-01-10 – 2015-01-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] IMAGING OF MUSTY-ODOR-PRODUCING FILAMENTOUS CYANOBACTERIA BY VARIOUS MICROSCOPIES2014

    • Author(s)
      K. Takemoto, M. Yoshimura, A. Yamamoto, S. Ichise, H. Namba and H. Kihara
    • Organizer
      International Conference on X-Ray Microscopy 2014 (XRM2014)
    • Place of Presentation
      elbourne Convention and Exhibition Centre (Melbourne, Australia)
    • Year and Date
      2014-10-26 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] THREE-DIMENSIONAL OBSERVATION OF SOFT X-RAY MICROSCOPE AT THE SR CENTER OF RITSUMEIKAN UNIVERSITY2014

    • Author(s)
      M.Yoshimura, K.Takemoto, T.Ohigashi, H.Namba, H.Kihara, and T.Ohta
    • Organizer
      International Conference on X-Ray Microscopy 2014 (XRM2014)
    • Place of Presentation
      elbourne Convention and Exhibition Centre (Melbourne, Australia)
    • Year and Date
      2014-10-26 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 結像軟X線CTによる3次元構造観察2014

    • Author(s)
      大東 琢治, 吉村 真史, 竹本 邦子, 難波 秀俊, 木原 裕
    • Organizer
      日本バイオイメージング学会
    • Place of Presentation
      大阪大学銀杏会館 (大阪府吹田市)
    • Year and Date
      2014-09-04 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] X線顕微鏡によるPhormidium tenueとされている琵琶湖産糸状シアノバクテリアの微細構造観察2014

    • Author(s)
      竹本邦子, 吉村真史, 一瀬 諭, 難波秀利, 木原 裕
    • Organizer
      日本放射光学会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 軟X線顕微鏡の高度化と現状2014

    • Author(s)
      吉村真史, 竹本邦子, 木原裕, 難波秀利, 太田俊明
    • Organizer
      日本放射光学会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] hormidium tenueとされている琵琶湖産糸状藍藻の微細構造観察 ― XM,TEM,低真空クライオSEMによる ―2013

    • Author(s)
      本邦子, 山本章嗣, 一瀬 諭, 吉村真史, 塩野正道, 西村雅子, 水田 剛, 難波秀利, 木原 裕
    • Organizer
      日本水処理生物学会
    • Place of Presentation
      水道局たちばな職員研修センター(神戸市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 軟X線顕微鏡によるカビ種物質産生藍藻Phormidium tenueの微細構造観察2013

    • Author(s)
      竹本邦子, 一瀬 諭, 吉村真史, 難波秀利, 木原 裕
    • Organizer
      日本バイオイメージング学会
    • Place of Presentation
      東京大学薬学部講堂
    • Related Report
      2013 Research-status Report
  • [Presentation] 結像型軟X線顕微鏡試料システム2013

    • Author(s)
      竹本邦子, 吉村真史, 大東琢治, 臼井規真, 難波秀利, 木原 裕
    • Organizer
      日本放射光学会
    • Place of Presentation
      名古屋大学(愛知県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 軟X線顕微鏡によるピコ植物プランクトンの粘質鞘の解析2012

    • Author(s)
      竹本邦子, 一瀬 諭, 池谷仁里, 大東琢治, 難波秀利, 木原裕
    • Organizer
      日本バイオイメージング学会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 軟X線顕微鏡による糸状植物プランクトンの細胞内顆粒の観察2012

    • Author(s)
      竹本邦子, 山本章嗣, 水田 豪, 吉村真史, 一瀬 諭, 難波秀利, 木原 裕
    • Organizer
      日本バイオイメージング学会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2012 Research-status Report
  • [Presentation] Soft X-ray Imaging of Intracellular Granules in Filamentous Cyanobacterium2012

    • Author(s)
      Kuniko Takemoto, Go Mizuta, Akitsugu Yamamoto, Masashi Yoshimura, Satoshi Ichise, Hidetoshi Namba and Hiroshi Kihara
    • Organizer
      11th International Conference on X-ray Microscopy
    • Place of Presentation
      Hope Hotel (Shanghai, China)
    • Related Report
      2012 Research-status Report
  • [Presentation] Improvement of Cryogenic 3-Dimensional Observation System of Soft X-ray Microscope at the SR Center of Ritsumeikan University2012

    • Author(s)
      Kuniko Takemoto, Kishin Usui, Takuji Ohigashi, Hiroki Fujii, Masashi Yoshimura, Hidetoshi Namba and Hiroshi Kihara
    • Organizer
      11th International Conference on X-ray Microscopy
    • Place of Presentation
      Hope Hotel (Shanghai, China)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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