Estimation of Organic Carbon Content of the Cyanobacterium by Soft X-ray Microscopy
| Project/Area Number |
24510039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Akitsugu 長浜バイオ大学, バイオサイエンス学部, 教授 (30174775)
IKEGAYA Hisato 兵庫県立大学, 理学部, 客員研究員 (30531579)
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| Research Collaborator |
ICHISE Satoshi 滋賀県琵琶湖環境科学研究センター, 環境監視部門, 専門員
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 軟X線顕微鏡 / 微細シアノバクテリア / 細胞外代謝産物(EPS) / Synechococcus sp. / 有機物量評価 / Ｘ線マイクロCT / 細胞外代謝産物 / シアノバクテリア / 軟Ｘ線顕微鏡 / 粒子性有機炭素 / X線マイクロCT / 炭素量換算 / 粒子性有機炭素(POC) |
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| Outline of Final Research Achievements |
Phytoplankton typically produces large amounts of extracellular polysaccharides (EPS), which is mainly composed of macromolecular heteropolysaccharides. As EPS contains large quantities of organic matter, it may be a major contributor to the increased COD in Lake Biwa. To confirm this speculation, a method for the accurate evaluation of the organic carbon (OC) content of phytoplankton by soft X-ray microscopy (XM) was developed and applied to Synechococcus sp. Soft XM is a non-destructive imaging technique that generates images based on differences in X-ray absorption by light elements, such as, carbon, oxygen, and nitrogen. Based on the X-ray absorption coefficients and gray levels of the XM images, the OC content of EPS-covered cells (0.39-0.47 pg/cell) was found to be 2.0-2.4 times larger than that of EPS-removed cells (0.20 pg/cell). These findings suggest that soft XM could be a useful tool for evaluating the OC content of picophytoplankton and EPS without pretreatment steps.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Estimation of Organic Carbon Content of the Cyanobacterium Synechococcus sp. by Soft X-ray Microscopy2015
Author(s)
H. Ikegaya, S. Suzuki, S. Ichise, S. Furuta, S. Wakabayashi, T. Ohigashi, D. Bamba, H. Namba, H. Kihara, N. Kishimoto, K. Takemoto
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Journal Title
Geomicrobiology Journal
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] 結像軟X線CTによる3次元構造観察2014
Author(s)
大東 琢治, 吉村 真史, 竹本 邦子, 難波 秀俊, 木原 裕
Organizer
日本バイオイメージング学会
Place of Presentation
大阪大学銀杏会館 （大阪府吹田市）
Year and Date
2014-09-04 – 2014-09-06
Related Report
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