Biochemical and dynamic structural analysis of translesion DNA polymerase
| Project/Area Number |
24510066
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Nagasaki University (2014)
Kumamoto University (2013)
Chiba University (2012) |
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Principal Investigator |
KARATA Kiyonobu 長崎大学, 原爆後障害医療研究所, 産学官連携研究員 (90345017)
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| Co-Investigator(Renkei-kenkyūsha) |
OGURA Teru 熊本大学, 発生医学研究所, 教授 (00158825)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | DNA複製 / 損傷乗り越え / 突然変異 / DNAポリメラーゼV / リボヌクレオチド |
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| Outline of Final Research Achievements |
DNA polymerase V is responsible for most mutations that arise from DNA damages in E. coli. We found that Pol V can incorporate ribonucleotide as well as deoxyribonucleotide and proposed a new model referred to as “ribonucleotide excision repair (RER)”. In the model, a part of Pol V-synthesized strand is resynthesized by error-free DNA polymerase utilizing ribonucleotide-deoxyribonucleotide hybrid region and it leads to a reduction of mutation frequency. We also found that RecA bound to UmuC, Pol V’s catalytic subunit, in 2 different regions. One was for active Pol V, and the other was for inactive Pol V.
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Report
(4 results)
Research Products
(9 results)
