| Project/Area Number |
24510078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Manabu 独立行政法人放射線医学総合研究所, 放射線障害研究グループ, 主任研究員 (70280740)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | DNA損傷修復 / DNA二本鎖切断 / Ku / 分子シミュレーション / ライブセルイメージング / DNA損傷認識 / 損傷修復 / 損傷認識 |
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| Outline of Final Research Achievements |
The binding mechanism of the repair enzyme, Ku, to DNA that has overhangs in its 5’- or 3’- end was examined by both computational and experimental techniques. Comparing with the interaction between Ku and DNA with blunt ends, no significant differences were observed in the binding energy between Ku and DNA, but an amino acid residue of Ku that would interact with the terminal region with single-stranded DNA was identified. In addition, we established the system to examine the dynamics of Ku70 and Ku80, subunits of Ku protein, in the nucleus of cultured cells using the live cell imaging method, and succeeded in observing the accumulation of Ku70 or Ku80 at DNA damage sites produced by laser-microirradiation.
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