| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
In the present study, we analyzed the effect of prenatal diesel exhaust (DE) exposure on the DNA methylation of neural stem cells (NSCs). Furthermore, we also analyzed the relationship between altered DNA methylation profile and the function of NSCs.
Pregnant mice were exposed to DE and NSCs were obtained from brains which collected from 1-day-old offspring. Genome-wide DNA methylation profile of NSCs was disrupted by prenatal DE exposure. In addition, altered expression of mRNAs which are related to neural differentiation or maintenance of stem cell phenotype was detected. The mRNA expression levels of neural differentiation markers in the NSCs were decreased by prenatal DE exposure.
These results suggested that disrupted DNA methylation induced by prenatal DE exposure altered the expression level of mRNA which related to differentiation of neural cells. Furthermore, altered expression of mRNA was associated with deregulation of neural differentiation during development period.
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