Structure-function studies of metabolic enzymes for design of novel biodegradable biopolymers

• Project/Area Number: 24510114
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HISANO Tamao 独立行政法人理化学研究所, 放射光科学総合研究センター, 専任研究員 (20312267)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 環境調和型材料 / バイオポリエステル / 生分解性高分子 / 微生物酵素 / 構造と機能 / 結晶構造解析 / 生分解性

Outline of Final Research Achievements

Polyhydroxyalkanoate (PHA) is important bacterial polyester that is degraded by specific enzymes in environment. In this study, structure and function relationships of enzymes in PHA metabolism were investigated for the sake of development of the design and synthetic system of new biopolyesters. A multi-domain PHA depolymerase was crystallized, and its crystal structure in complex with an oligomeric substrate was successfully determined. The results indicated a novel interaction mode between the PHA depolymerase with the type I catalytic domain and polymer substrate. In addition, a novel hydrolase which degrades a soluble polymer polyaspartate was successfully crystallized, and its crystal structure in complex with an oligomeric substrate were determined. The interaction mode between the enzyme and the substrate indicated similarity with that of a fungal PHA depolymerase with the type II catalytic domain.

Research Products

• [Presentation] ポリアスパラギン酸分解酵素の基質複合体の構造解析 (2014)
  • Author(s): 久野玉雄・平石知裕・南 皓介・桝田エリ子・阿部英喜・前田瑞夫・城 宜嗣
  • Organizer: 日本結晶学会平成２６年度年会
  • Place of Presentation: 東京大学弥生キャンパス
  • Year and Date: 2014-11-01 – 2014-11-03
• [Presentation] ポリアスパラギン酸分解酵素の結晶構造 (2014)
  • Author(s): 久野玉雄・平石知裕・南 皓介・桝田エリ子・阿部英喜・前田瑞夫・城 宜嗣
  • Organizer: 第６３回高分子討論会
  • Place of Presentation: 長崎大学文教キャンパス
  • Year and Date: 2014-09-25
• [Presentation] Enzymatic hydrolysis of the β－β amide linkage in poly-β-aspartate (2014)
  • Author(s): Tamao Hisano, Tomohiro Hiraishi, Kosuke Minami, Eriko Masuda, Hideki Abe, Mizuo Maeda, Yoshitsugu Shiro
  • Organizer: 23rd Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-08-06 – 2014-08-08
• [Presentation] ポリアスパラギン酸分解酵素の結晶構造
  • Author(s): 久野玉雄、平石知裕、南皓介、桝田エリ子、阿部英喜、前田瑞夫、城宜嗣
  • Organizer: 第１３回日本蛋白質科学会年会
  • Place of Presentation: とりぎん会館、鳥取
• [Presentation] Crystal structure of poly-b-aspartate hydrolase from Pedobactor sp. KP-2
  • Author(s): Tamao Hisano, Tomohiro Hiraishi, Kosuke Minami, Eriko Masuda, Hideki Abe, Mizuo Maeda, Yoshitsugu Shiro
  • Organizer: The 4th International Conference on Biobased Polymers (ICBP2013)
  • Place of Presentation: 漢陽大学校, Seoul, Korea
• [Presentation] ポリアスパラギン酸分解酵素の結晶構造解析
  • Author(s): 久野玉雄、平石知裕、南皓介、桝田エリ子、阿部英喜、前田瑞夫、城宜嗣
  • Organizer: 平成２５年度日本結晶学会年会
  • Place of Presentation: 熊本大学、熊本
• [Presentation] ポリ-β-アスパラギン酸分解酵素とオリゴマー基質との複合体の結晶構造
  • Author(s): 久野玉雄、平石知裕、南皓介、桝田エリ子、阿部英喜、前田瑞夫、城宜嗣
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド、神戸
• [Presentation] Introduction to Protein Crystallography
  • Author(s): Tamao Hisano, Tomohiro Hiraishi, Kosuke Minami, Eriko Masuda, Hideki Abe, Mizuo Maeda, Yoshitsugu Shiro
  • Organizer: Introduction to Protein Crystallography A Hands-On Workshop
  • Place of Presentation: Universiti Sains Malaysia, Penang, Malaysia
  • Invited