Traits improvement by ion-beam and gamma-ray irradiation in entomopathogenic fungi

• Project/Area Number: 24510121
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Quantum beam science
• Research Institution: Shizuoka University
• Principal Investigator: SAITO Tsutomu 静岡大学, (連合)農学研究科(研究院), 教授 (40402234)
• Co-Investigator(Kenkyū-buntansha): NARUMI Issey 独立行政法人日本原子力研究開発機構, 研究主幹 (90343920) ; SATOH Katsuya 独立行政法人日本原子力研究開発機構, 研究員 (90370402)
• Research Collaborator: SHINOHARA Shinobu ; FITRIANA Yuyun
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 昆虫病原糸状菌 / イオンビーム / ガンマ線 / 殺菌剤耐性 / 高温耐性 / 改良 / 突然変異

Outline of Final Research Achievements

Entomopathogenic fungi, such as Isaria fumosorosea, Beauveria bassiana and Metarhizium anisopliae, are important agents in IPM systems of insect pests, however they are not compatible with fungicides and high temperatures. This study indicated that ion-beam and gamma ray irradiation technology successfully produced fungicide resistant mutants and thermotolerant mutants. The technology has great potential as a tool to improve the traits of entomopathogenic fungi.

Research Products

• [Journal Article] Benomyl‑resistant Beauveria bassiana (Hypocreales: Clavicipitaceae) mutants induced by ion beams (2015)
  • Author(s): Yuyun Fitriana, Shinobu Shinohara, Katsuya Satoh, Issay Narumi & Tsutomu Saito
  • Journal Title: Applied Entomology and Zoology Volume: 50 Issue: 1 Pages: 123-129
  • DOI: 10.1007/s13355-014-0314-7
  • NAID: 120005690326
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ion-beam and gamma-ray irradiations induce thermotolerant mutants in the entomopathogenic fungus Metarhizium anisopliae s.l. (2014)
  • Author(s): Yuyun Fitriana, Katsuya Satoh, Issay Narumi & Tsutomu Saito
  • Journal Title: Biocontrol Science and Technology Volume: 24 Issue: 9 Pages: 1052-1061
  • DOI: 10.1080/09583157.2014.918585
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Enhanced fungicide resistance in Isaria fumosorosea following ionizing radiation-induced mutagenesis (2013)
  • Author(s): Shinobu Shinohara, Yuyun Fitriana, Kastuya Satoh, Issay Narumi, and Tsutomu Saito
  • Journal Title: FEMS Microbiology Letters Volume: 349 Pages: 54-60
  • DOI: 10.1111/1574-6968.12295
  • Peer Reviewed
• [Presentation] 量子ビーム照射によって作出された昆虫寄生菌殺菌剤耐性変異株の遺伝子解析 (2013)
  • Author(s): 西東力, Yuyun Fitriana, 佐藤勝也, 鳴海一成
  • Organizer: 第8回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎市（群馬県）
• [Remarks] 第7回 高崎両市応用研究シンポジウム
  • URL: http://www.taka.jaea.go.jp/tiara/j661/symposium/sympo_7/7sympo_prog.pdf