| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Outline of Final Research Achievements |
Assembling mechanism of platinum nanoparticle (PtNP) into a lysozyme crystal was examined for two different types of the PtNPs which have either positive or negative zeta potential. The distribution coefficient of the positive ones inside the crystal is much higher than that of the negative ones. Dispersivity of the PtNPs before interaction with the lysozyme crystal surface causes a profound difference on the assembly of the PtNPs into the crystal. Moreover, an effect of PtNP as additive on protein crystal nucleation was investigated. The presence of the PtNPs increased number of crystals per drop. PtNPs have two roles in the nucleation of lysozyme, condensation and charge delocalization of lysozyme.
Fluorescence microscope for investigation of the dynamics of protein crystallization was manufactured. Process of crystallization can be observed for several days by using this optical system, which can detects fluorescence of single nanoparticles.
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