An analysis of bacteria, which produce 'Biotech-materials'

• Project/Area Number: 24510151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Okayama University
• Principal Investigator: KASAI Tomonari 岡山大学, 自然科学研究科, 講師 (30530191)
• Co-Investigator(Kenkyū-buntansha): SENO Masaharu 岡山大学, 大学院自然科学研究科, 教授 (90243493) ; HASHIMOTO Hideki 岡山大学, 大学院自然科学研究科, 助教 (60579556)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 三次元細胞培養 / ドラッグデリバリーシステム / 酸化鉄マイクロチューブ / ヒト癌由来細胞株 / チューブ状酸化鉄 / L-BIOX / HepG2細胞 / 細胞塊 / 多孔質構造 / シリカ / 鉄酸化細菌 / ＤＤＳ / 細胞培養 / 培養基材 / 分子標的 / クロロトキシン

Outline of Final Research Achievements

We developed an application for 3D cell culture substrate using amorphous iron oxide (L-BIOX). This culture system should be a promising the production of proteins. On the other hand, to develop the application for drug delivery system, we studied protein ligands and anti-cancer drugs for targeting the cancer cells.

Research Products

• [Journal Article] Characterization of cancer stem-like cells derived from mouse induced pluripotent stem cells transformed by tumor-derived extracellular vesicles (2014)
  • Author(s): Yan T, Mizutani A, Chen L, Takaki M, Hiramoto Y, Matsuda S, Shigehiro T, Kasai T, Kudoh T, Murakami H, Masuda J, Hendrix MJ, Strizzi L, Salomon DS, Fu L, Seno M.
  • Journal Title: Journal of Cancer Volume: 5 Issue: 7 Pages: 572-584
  • DOI: 10.7150/jca.8865
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Efficient drug delivery of Paclitaxel glycoside: a novel solubility gradient encapsulation into liposomes coupled with immunoliposomes preparation. (2014)
  • Author(s): Shigehiro T, Kasai T, Murakami M, Sekhar SC, Tominaga Y, Okada M, Kudoh T, Mizutani A, Murakami H, Salomon DS, Mikuni K, Mandai T, Hamada H, Seno
  • Journal Title: PLoS One Volume: 9 Issue: 9 Pages: e107976-e107976
  • DOI: 10.1371/journal.pone.0107976
  • NAID: 120005477121
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] In vitro anti-proliferative and anti-angiogenic activities of thalidomide dithiocarbamate analogs. (2014)
  • Author(s): El-Aarag BY, Kasai T, Zahran MA, Zakhary NI, Shigehiro T, Sekhar SC, Agwa HS, Mizutani A, Murakami H, Kakuta H, Seno M.
  • Journal Title: International Immunopharmacology Volume: 2 Issue: 2 Pages: 283-92
  • DOI: 10.1016/j.intimp.2014.05.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cancer stem cells converted from pluripotent stem cells and the cancerous niche. (2014)
  • Author(s): Kasai T, Chen L, Mizutani A, Kudoh T, Murakami H, Fu L, Seno M
  • Journal Title: Journal of Stem Cells & Regenerative Medicine Volume: 1 Pages: 2-7
  • Peer Reviewed / Open Access
• [Journal Article] Chlorotoxin-Fc Fusion Inhibits Release of MMP-2 from Pancreatic Cancer Cells. (2014)
  • Author(s): El-Ghlban S, Kasai T, Shigehiro T, Yin HX, Sekhar S, Ida M, Sanchez A, Mizutani A, Kudoh T, Murakami H, Seno M
  • Journal Title: Biomed Res Int. Volume: - Pages: 152659-152659
  • DOI: 10.1155/2014/152659
  • Peer Reviewed / Open Access
• [Journal Article] Identification of caveolin-1 as a potential causative factor in the generation of trastuzumab resistance in breast cancer cells. (2013)
  • Author(s): Sekhar SC, Kasai T, Satoh A, Shigehiro T, Mizutani A, Murakami H, El-Aarag BY, Salomon DS, Massaguer A, de Llorens R, Seno M
  • Journal Title: J Cancer Volume: 4 Issue: 5 Pages: 391-401
  • DOI: 10.7150/jca.6470
  • Peer Reviewed
• [Journal Article] Chlorotoxin Fused to lgG・Fc Inhibits Glioblastoma Cell Motility via Receptor-Mediated Endocytosis. (2012)
  • Author(s): T. Kasai and M. Okada, et al.
  • Journal Title: Journal of Drug Delivery Volume: 2012 Pages: 1-10
  • DOI: 10.1155/2012/975763
  • Peer Reviewed
• [Presentation] ヒトiPS 細胞から作るがん幹細胞 (2014)
  • Author(s): 笠井智成、水谷昭文、工藤孝幸、佐藤あやの、陳凌、妹尾昌治
  • Organizer: 第37 回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] iPS 細胞から作るがん幹細胞ライブラリー-抗がん剤スクリーニングプラットフォームとして- (2014)
  • Author(s): 笠井智成、本庶仁子、公文一輝、星川健太、竹尻崇人、朝倉真実、水谷昭文、村上宏、妹尾昌治
  • Organizer: 化学療法基盤支援活動第3 回シンポジウム
  • Place of Presentation: 沖縄
  • Year and Date: 2014-05-12
• [Presentation] Anti-cancer activity of immunoliposomes encapsulated effective amount of glycosylated paclitaxel with novel loading strategy (2014)
  • Author(s): Tsukasa Shigehiro, Tomonari Kasai, Akifumi Mizutani, Hiroshi Murakami, Katsuhiko Mikuni, Tadakatsu Mandai, Hiroki Hamada, Masaharu Seno
  • Organizer: American Association for Cancer Research Annual Meeting 2014
  • Place of Presentation: San Diego, CA
• [Presentation] Chlorotoxin-Fc fusion inhibits release of MMP-2 from pancreatic cancer cells (PANC-1) (2013)
  • Author(s): EL-Ghlban S., Kasai T., Kanao S., Mizutani A., Murakami H., Seno M.
  • Organizer: 第３６回 日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] ヒアルロン酸を用いたCD44高発現細胞の濃縮 (2013)
  • Author(s): 位田弥希子、笠井智成、村上宏、水谷昭文、妹尾昌治
  • Organizer: 第３６回 日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 高効率に糖修飾パクリタキセルを封入したイムノリポソームによる抗がん活性評価 (2013)
  • Author(s): 重廣司、笠井智成、富永有貴、水谷昭文、村上宏、三國克彦、萬代忠勝、濱田博喜、妹尾昌治
  • Organizer: 第３６回 日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] A novel remote loading method with solubility gradient to encapsulate effevtive amount of taxanes into liposomes. (2013)
  • Author(s): T. Shigehiro, T. Kasai, A. Mizutani, H. Murakami, K. Mikuni, T. Mandai, H. Hamada, M. Seno.
  • Organizer: American association for cancer research AACR annual meeting 2013
  • Place of Presentation: Washington, DC
• [Presentation] In vitro niche created by cancer stem-like cells derived from mouse induced pluripotent stem cell (2013)
  • Author(s): A. Mizutani, S. Matsuda, T. Yan, T. Sota, T. Kasai, T. Kudoh, H. Murakami, M. Seno.
  • Organizer: American association for cancer research AACR annual meeting 2013
  • Place of Presentation: Washington, DC
• [Presentation] A new three dimensional culturing system to obtain scarce proteins and peptides (2012)
  • Author(s): Y.Tomoda, T.Kasai, Y.Miyawaki, H.Hashimoto, T.Suzuki, T.Kudoh, J.Takada, M.Seno
  • Organizer: 日本分子生物学会 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Development of the system to target CD44 protein for treatment of glioma (2012)
  • Author(s): M.Ida, T.Kasai, Y.Sugii, T.Kudoh, A.Mizutani, H.Murakami, M.Seno
  • Organizer: 日本分子生物学会 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Preparation and evaluation of HER2 targeting immunoliposomes with paclitaxel glycoside (2012)
  • Author(s): T.Shigehiro, T.Kasai, T.Matsumoto, M.Okada, A.Mizutani, T.Kudoh, H.Murakami, K.Mikuni, T.Mandai, H.Hamada, M.Seno
  • Organizer: 日本分子生物学会 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Influence of caveolin-1 in ligand based targeting of ErbB2 in breast cancer cell line SKBR3 (2012)
  • Author(s): S.Sekhar, T.Kasai, T.Hashizume, T.Kudoh, A.Mizutani, A.Satoh, H.Murakami, M.Seno
  • Organizer: 日本分子生物学会 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] パクリタキセルグリコシド内封リポソームによるHER2過剰発現がん細胞の標的 (2012)
  • Author(s): 重廣司、笠井智成、岡田将史、水谷昭文、工藤孝幸、三國克彦、萬代忠勝、妹尾昌治
  • Organizer: 日本癌学会 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Remarks] ナノバイオシステム分子設計学 妹尾研究室
  • URL: http://www.cyber.biotech.okayama-u.ac.jp/senolab/