| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Outline of Final Research Achievements |
In this project, we developed a novel high-throughput screening and analysis system for circulating tumor cells (CTCs) using a cell microarray chip. The cell microarray chip with 20,944 microchambers (105 micrometer diameter) was made from polystyrene by using LIGA process. The chip surface was rendered to hydrophilic by means of reactive-ion etching, which led to the mono-layers formation of leukocytes. Leukocytes isolated from whole blood were dispersed on the chip surface, followed by 15 min standing. Approximately 89 leukocytes entered each microchamber when about 1,800,000 leukocytes in total were placed onto the cell microarray chip. In the experiments using spiked NCI-H1650 cells (0.01 to 0.0001%), accurate detection of carcinoma cells was achieved with monoclonal antibodies. Further, we could retrieve target carcinoma cells by micromanipulator for DNA analysis. Using the cell microarray chip system, we also succeed to detect CTCs in peripheral blood of lung cancer patients.
|
