| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Chronological lifespan (CLS) is defined as the length of time a cell can survive without cell division, which may serve as a good model for studying the mechanisms regulating life span of differentiated cells that are generally non-dividing. Chronological age of cells has been estimated by measuring colony forming units of cells in stationary phase. However, this method is a very time-consuming and daunting task. To address this issue, we tried to develop molecular markers that can provide an indirect measure of chronological age of a cell using fission yeast. Using the strain collection, a set of strains that can express each of the fission yeast ORFs with small epitope tags, we identified proteins whose expression levels were altered according to the time in stationary phase. Tagging of these candidates with fluorescent proteins allows immediate measuring of cell age from the fluorescence intensity. These aging markers will help identify factors regulating CLS.
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