The function of the novel noncoding RNA related to the growth control of myeloid leukemia cells

• Project/Area Number: 24510277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Hiroshima University
• Principal Investigator: HIRANO Tetsuo 広島大学, 総合科学研究科, 助教 (50228805)
• Co-Investigator(Renkei-kenkyūsha): HARADA Hironori 順天堂大学, 医学部, 准教授 (10314775) ; HARADA Yuka 順天堂大学, 医学部, 助教 (50379848) ; ISHIDA Atsuhiko 広島大学, 総合科学研究科, 教授 (90212886)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 機能性非コードRNA / 骨髄性白血病 / 増殖制御 / 血清飢餓 / 遺伝子ノックダウン / shRNA / 受容体型チロシンキナーゼ / KIT / ノックダウン / チロシンキナーゼ型受容体 / 非コード性RNA / 疾患関連遺伝子 / 染色体外因子

Outline of Final Research Achievements

A long noncoding RNA, CCDC26, which is reportedly the most common gene whose copy number is altered in pediatric acute myeloid leukemia cells. We identified some transcripts abundant in myeloid leukemia cells within the CCDC26 genetic locus, and the concentration of these transcripts in the nuclei of the cells. Knock down of CCDC26 resulted in the prolonged survival period of the cells in the culture medium without serum. The expression of KIT, a well known oncogene functioning as a receptor tyrosine kinase, was significantly enhanced in the CCDC26-knock down cells, suggesting that this gene controls proliferation of the myeloid leukemia cells through KIT expression. These result might give an impact to the therapy strategy for myeloid leukemia with mutated CCDC26 genes.

Research Products

• [Journal Article] Long noncoding RNA, CCDC26, controls myeloid leukemia cell growth through regulation of KIT expression (2015)
  • Author(s): Hirano Tetsuo, Yoshikawa Ryoko, Harada Hironori, Harada Yuka, Ishida Atsuhiko, Yamazaki Takeshi
  • Journal Title: Molecular Cancer Volume: 14 Issue: 1 Pages: 90-104
  • DOI: 10.1186/s12943-015-0364-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Is CCDC26 a novel cancer-associated long-chain non-coding RNA? (2013)
  • Author(s): Tetsuo Hirano
  • Journal Title: Oncogene and Cancer from bench to clinic edited by Yahwardiah Siregar Volume: not available Issue: 8
  • DOI: 10.5772/54966
  • Peer Reviewed
• [Presentation] 非コードRNA CCDC26がc-Kitの転写調節を介して骨髄性白血病細胞の増殖を制御している (2014)
  • Author(s): 平野哲男、吉川量子、原田浩徳、原田結花、石田敦彦、山崎岳
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-27
• [Presentation] 急性骨髄性白血病に関係するCCDC26のマウスホモログ同定と発現解析 (2014)
  • Author(s): 吉川量子、原田浩徳、原田結花、石田敦彦、山崎岳、平野哲男
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-27