Chemical and Biological Studies of the Bioactive Supermolecules from Red Tide

• Project/Area Number: 24510298
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Hiroshima Institute of Technology
• Principal Investigator: Yoshikazu Hiraga 広島工業大学, その他部局等, 教授 (10238347)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 赤潮プランクトン / ヘテロカプサ / 構造解析 / 構造活性相関 / 機器分析

Outline of Final Research Achievements

In order to elucidate the chemical structure and biological activity of the bioactive supermolecule from the harmful phytoplankton, Heterocapsa circularisquama caused mass mortalities of bivalves, e.g. oyster, the purification of the active compounds and the chemical degradation reactions were carried out. Also, in order to evaluate of the fatty acid composition of the H. circularisquama, the extract of the algae was analyzed. Major cnstituents of fatty acid were saturated C14 and C16 fatty acid.

Research Products

• [Journal Article] Marangoni flow around a camphor disk regenerated by the interaction between camphor and sodium dodecyl sulfate molecules (2015)
  • Author(s): S. Nakata, R. Tenno, A. Deguchi, H. Yamamoto, Y. Hiraga, S. Izumi
  • Journal Title: Colloids and Surfaces A: Physicochemical and Engineering Aspects Volume: 466 Pages: 40-44
  • DOI: 10.1016/j.colsurfa.2014.10.041
  • Peer Reviewed / Open Access
• [Journal Article] Formation of isomerized E,Z-configured 1,3-dienes in construction of macrocyclic trienes by diene-ene RCM (2015)
  • Author(s): R. Takagi, K. Tanaka, K. Yamamoto, Y. Hiraga, S. Kojima, M. Abe
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 88 Issue: 1 Pages: 146-148
  • DOI: 10.1246/bcsj.20140250
  • NAID: 130004690771
  • Peer Reviewed / Open Access
• [Journal Article] The mthA mutation conferring low-level resistance to streptomycin enhances antibiotic production in Bacillus subtilis by increasing the S-adenosylmethionine pool size (2014)
  • Author(s): Tojo S, Kim JY, Tanaka Y, Inaoka T, Hiraga Y, Ochi K.
  • Journal Title: J Bacteriol Volume: 196 Issue: 8 Pages: 1514-1524
  • DOI: 10.1128/jb.01441-13
  • Peer Reviewed
• [Journal Article] 生物工学とケミカルバイオロジー (2013)
  • Author(s): 平賀良知
  • Journal Title: 生物工学会誌 Volume: 91 Pages: 526-526
  • Peer Reviewed
• [Journal Article] A novel non-metathetic behavior of Grubbs catalyst: Ruthenium-mediated intramolecular [3+2] cycloaddition of bis-1,3-dienes (2013)
  • Author(s): Ryukichi Takagi, Koumei Yamamoto, Yoshikazu Hiraga, Satoshi Kojima, Manabu Abe
  • Journal Title: Journal of Organometallic Chemistry Volume: 723 Pages: 171-175
  • DOI: 10.1016/j.jorganchem.2012.09.004
  • Peer Reviewed
• [Presentation] Mechanistic Studies on Selective Monohydrolysis of Symmetric Diesters with the use of Dynamic Light Scattering/Electrophoretic Light Scattering (2016)
  • Author(s): Satomi Niwayama and Yoshikazu Hiraga
  • Organizer: 日本化学会 第96春季年会
  • Place of Presentation: 同志社大学 京田辺キャンパス(京都府京田辺市）
  • Year and Date: 2016-03-26
• [Presentation] 放線菌・枯草菌における低レベルストレプトマイシン耐性変異の発見とその利用 (2014)
  • Author(s): 越智幸三・東條繁朗・金 智潤・田中幸徳・稲岡隆史・平賀良知
  • Organizer: 第29回日本放線菌学会
  • Place of Presentation: つくばカピオ(茨城県つくば市）
  • Year and Date: 2014-06-19 – 2014-06-20