| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Outline of Final Research Achievements |
Based on the production of IFN-γ from the cultivated splenocytes, I aimed to identify immunoregulatory factors from liver sinusoidal endothelial cells (LSEC) and cancer cells. I succeeded to isolate and purify LSEC in more than 90% with magnet beads. I discovered that the production of IFN-γin the cultured splenocytes from the 4T1 tumor-bearing mouse is transiently upregulated and then decreased, and further suppressed by 4T1-conditioned medium. The purification of the suppressive factors from the 4T1-conditioned medium succeeded in the identification of G-CSF and M-CSF. The suppressive factors in the CT26-conditioned medium seemed to be smaller than 10kDa, therefor; these were analyzed by HPLC and LC-MS and succeeded in to show PGE2 in the conditioned medium. It is hoped that various immunosuppressive substances are identified from this assay system.
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