Search for immunoregulatory factors derived from LSECs and Cancer cells
Project/Area Number |
24510300
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Living organism molecular science
|
Research Institution | Kyushu University |
Principal Investigator |
KANO Arihiro 九州大学, 先導物質化学研究所, 准教授 (30403950)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
Keywords | がん / 腫瘍 / 免疫抑制 / IFN-γ / 免疫 / ガン細胞 / 液性因子 / 肝類洞内皮細胞 / 免疫抑制因子 |
Outline of Final Research Achievements |
Based on the production of IFN-γ from the cultivated splenocytes, I aimed to identify immunoregulatory factors from liver sinusoidal endothelial cells (LSEC) and cancer cells. I succeeded to isolate and purify LSEC in more than 90% with magnet beads. I discovered that the production of IFN-γin the cultured splenocytes from the 4T1 tumor-bearing mouse is transiently upregulated and then decreased, and further suppressed by 4T1-conditioned medium. The purification of the suppressive factors from the 4T1-conditioned medium succeeded in the identification of G-CSF and M-CSF. The suppressive factors in the CT26-conditioned medium seemed to be smaller than 10kDa, therefor; these were analyzed by HPLC and LC-MS and succeeded in to show PGE2 in the conditioned medium. It is hoped that various immunosuppressive substances are identified from this assay system.
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Report
(4 results)
Research Products
(3 results)