| Project/Area Number |
24510301
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
SAKAMOTO Hiroshi 九州工業大学, 大学院情報工学研究院, 教授 (70309748)
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| Co-Investigator(Kenkyū-buntansha) |
YASUNAGA Takuo 九州工業大学, 大学院情報工学研究院, 教授 (60251394)
KOMATSU Hideyuki 九州工業大学, 大学院情報工学研究院, 助教 (90253567)
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| Co-Investigator(Renkei-kenkyūsha) |
NOGUCHI Masato 久留米大学, 医学部, 教授 (10124611)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ヘム / ヘムオキシゲナーゼ / シトクロムP450還元酵素 / 蛋白質間相互作用 / タンパク質間相互作用 / カロリメトリー / 触媒機構 |
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| Outline of Final Research Achievements |
Heme oxygenase (HO) catalyzes the O2-dependent degradation of heme using reducing equivalents from NADPH-cytochrome P450 reductase (CPR) and produces biliverdin, CO, and iron. Without reducing reagents, HO does not exhibit heme degradation activity but retains high substrate affinity to form a stable complex with heme. The heme bindings of wild-type HO-1 and its mutants were analyzed using isothermal titration calorimetry. Heme regulatory motifs at the C terminus of HO-2 were synthesized and their heme binding properties were investigated.
For electron transfer as well as product release, HO and CPR should repeat a cycle of association and dissociation, thus there might be some kind of regulation of protein-protein interaction between them. Using surface plasmon resonance we identified surface amino acids of HO-2 essential for binding to and/or electron transfer CPR. Furthermore, equilibrium associative properties between HO-1 and CPR were determined by analytical ultracentrifugation.
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