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Approaches for elucidation of structure-function relationships of modular chitinases using a novel modeling method

Research Project

Project/Area Number 24510302
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Living organism molecular science
Research InstitutionIwate Medical University

Principal Investigator

NONAKA Takamasa  岩手医科大学, 薬学部, 教授 (30242457)

Co-Investigator(Kenkyū-buntansha) KEZUKA Yuichiro  岩手医科大学, 薬学部, 助教 (50397163)
Co-Investigator(Renkei-kenkyūsha) SEKI Yasutaka  岩手医科大学, 薬学部, 准教授 (30377220)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsキチナーゼ / 抗真菌活性 / モデリング / 溶液散乱
Outline of Final Research Achievements

A bacterial chitinase (Chitinase C) is composed of two functional domains connected by a flexible linker. We prepared two kinds of mutant enzyme whose linker is elongated or replaced, and measured their antifungal and hydrolyzing activities. Although mutations in the linker were expected to vary the relative orientation of two domains, they affected the activities by up to only 25%. Furthermore, we developed a novel method to model protein structures with flexible interdomain linkers and select model structures as conformational ensembles on the basis of solution X-ray scattering data. The chitinase structures selected by this method can explain an action in chitin hydrolyzing we proposed previously.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 2013 2012

All Journal Article (1 results) Presentation (4 results)

  • [Journal Article] Structure of full-length class I chitinase from rice revealed by X-ray crystallography and small-angle X-ray scattering2013

    • Author(s)
      Yuichiro Kezuka
    • Journal Title

      Photon Factory Activity Rep. Part B

      Volume: 30 Pages: 270-270

    • Related Report
      2013 Research-status Report
  • [Presentation] モジュラーキチナーゼの立体構造と抗真菌活性の相関解明への試み2015

    • Author(s)
      毛塚雄一郎、関安孝、野中孝昌
    • Organizer
      第15回日本蛋白質科学会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2015-06-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] モジュラーキチナーゼの立体構造と抗真菌活性の相関解明への試み2014

    • Author(s)
      毛塚雄一郎、関安孝、野中孝昌
    • Organizer
      第8回東北糖鎖研究会
    • Place of Presentation
      岩手医科大学(岩手県紫波郡矢巾町)
    • Year and Date
      2014-10-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 蛋白質と糖鎖の相互作用2014

    • Author(s)
      野中孝昌
    • Organizer
      第8回東北糖鎖研究会
    • Place of Presentation
      岩手医科大学(岩手県紫波郡矢巾町)
    • Year and Date
      2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新しいペプチド鎖生成法を使用したIDPの分子モデリング2012

    • Author(s)
      関安孝
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋市
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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