Analysis of acquisition process of tyrosine kinase during eykaryotic evolution.

• Project/Area Number: 24510307
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Toho University
• Principal Investigator: KAWATA Takefumi 東邦大学, 理学部, 教授 (30221899)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: チロシンキナーゼ / シグナル伝達 / 転写因子 / SH2ドメイン / 細胞性粘菌 / チロシンキナーゼ様タンパク質

Outline of Final Research Achievements

Social amoeba, Dictyostelium discoideum, harbors a number of tyrosine kinase-like kinases (TKLs). In this project, we analyzed three TKLs, DrkA, DrkC and RckA, that may phosphorylate tyrosine residue on a transcription factor STATa. We created a strain with a mutation of all three genes. The phosphorylation of the STATa was almost abolished in the mutant strain using a buffered artificial phosphorylation induction experiment. This indicates these TKLs play vital roles for STATa phosphorylation. However, STATa was phosphorylated in the mutant strain when it developed into a multicellular stage. Although we cannot explain this paradox at the moment, if the function of any particular TKL is inactivated then other TKL including unidentified novel one might replace it to show redundancy. To elucidate the redundancy mechanism would help to understand the new insights into evolutional acquisition process of protein tyrosine kinase.

Research Products

• [Journal Article] Regulation of ecmF gene expression and genetic hierarchy among STATa, CudA, and MybC on several prestalk A-specific gene expressions in Dictyostelium. (2016)
  • Author(s): Saga, Y., Inamura, T., Shimada, N. and Kawata, T.
  • Journal Title: Development Growth & Differentiation Volume: 印刷中 Issue: 4 Pages: 383-399
  • DOI: 10.1111/dgd.12285
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Implications of expansin-like 3 gene in Dictyostelium morphogenesis. (2015)
  • Author(s): Kawata, T., Nakamura, Y., Saga, Y., Iwade, Y., Ishikawa, M., Sakurai, A. and Shimada, N.
  • Journal Title: SpringerPlus Volume: 4 Issue: 1 Pages: 190-190
  • DOI: 10.1186/s40064-015-0964-0
  • Peer Reviewed / Open Access
• [Journal Article] Two Dictyostelium tyrosine kinase-like kinases function in parallel, stress-induced STAT activation pathways. (2014)
  • Author(s): Araki, T., Vu, L.H. Sasaki, N., Kawata, T., Eichinger, L. and Williams, J.G.
  • Journal Title: Molecular Biology of the Cell Volume: 25 Issue: 20 Pages: 3222-3233
  • DOI: 10.1091/mbc.e14-07-1182
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A Dictyostelium cellobiohydrolase orthologue that affects developmental timing. (2014)
  • Author(s): Kunii, M., Yasuno, M., Shindo, Y. and Kawata, T.
  • Journal Title: Development Genes Evolution Volume: 224 Issue: 1 Pages: 25-35
  • DOI: 10.1007/s00427-013-0460-x
  • Peer Reviewed
• [Journal Article] Identification of the kinase that activates a non-metazoan STAT gives insights the evolution of phosphotyrosine-SH2 domain signaling. (2012)
  • Author(s): Araki, T., Kawata, T. and Williams, J.G.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 109 Issue: 28
  • DOI: 10.1073/pnas.1202715109
  • Peer Reviewed
• [Presentation] オーガナイザー特異的遺伝子ecmFにおけるSTATa作用領域の同定 (2015)
  • Author(s): 嵯峨幸夏、稲村友香、島田奈央、川田健文
  • Organizer: 第５回日本細胞性粘菌学会
  • Place of Presentation: 弘前大学（青森県弘前市）
  • Year and Date: 2015-10-10
• [Presentation] TLKキナーゼDrkCの機能解析 (2015)
  • Author(s): 石川恵、荒川今日子、川田健文
  • Organizer: 第５回日本細胞性粘菌学会
  • Place of Presentation: 弘前大学（青森県弘前市）
  • Year and Date: 2015-10-10
• [Presentation] Analyses of TKL kinase, DrkA, in STATa tyrosine phosphorylation. (2015)
  • Author(s): 嵯峨幸夏、岩出結実、石川恵、荒木剛、Jeffrey G. Williams、川田健文
  • Organizer: 第５回日本細胞性粘菌学会
  • Place of Presentation: 弘前大学（青森県弘前市）
  • Year and Date: 2015-10-10
• [Presentation] Analyses of DrkA kinase in STATa activation. (2015)
  • Author(s): Saga, Y., Iwade, Y., Ishikawa, M., Araki, T., Williams, J.G. and Kawata, T.
  • Organizer: Dicty 2015 Annual International Dictyostelium Conference
  • Place of Presentation: London, UK
  • Year and Date: 2015-08-11
  • Int'l Joint Research
• [Presentation] TKLキナーゼDrkAのSTATaリン酸化能について (2014)
  • Author(s): 岩出結実，荒木剛、Jeffrey G. Williams，川田健文
  • Organizer: 第４回日本細胞性粘菌学会
  • Place of Presentation: 東北大学片平さくらホール(宮城県仙台市)
  • Year and Date: 2014-10-11
• [Presentation] Two Dictyostelium tyrosine kinase-like kinase function in parallel, stress-induced STAT activation pathways. (2014)
  • Author(s): Tsutyoshi Araki, Linh Hai Vu, Norimitsu Sasaki, Takefumi Kawata, Ludwig Eichinger and Jeffrey G. Williams
  • Organizer: 第４回日本細胞性粘菌学会
  • Place of Presentation: 東北大学片平さくらホール(宮城県仙台市)
  • Year and Date: 2014-10-11
• [Presentation] 多細胞期におけるTKLキナーゼDrkCの機能について (2014)
  • Author(s): 石川恵、荒川今日子、荒木剛、Jeffrey G. Williams，川田健文
  • Organizer: 第４回日本細胞性粘菌学会
  • Place of Presentation: 東北大学片平さくらホール(宮城県仙台市)
  • Year and Date: 2014-10-11
• [Presentation] STATa依存的な発現を示すオーガナイザー特異的遺伝子の階層性について (2014)
  • Author(s): 嵯峨幸夏、稲村友香、森川直樹、池羽好夢、島田奈央、川田健文
  • Organizer: 第４回日本細胞性粘菌学会
  • Place of Presentation: 東北大学片平さくらホール(宮城県仙台市)
  • Year and Date: 2014-10-11
• [Presentation] Two Dictyostelium Tyrosine Kinase-­‐Like kinases function in parallel, stress-­‐induced STAT activation pathways (2014)
  • Author(s): Tsuyoshi Araki, Linh Hai Vu, Norimitsu Sasaki, Takefumi Kawata, Ludwig Eichinger, Jeffrey G. Williams
  • Organizer: Dicty 2014 Annual International Dictyostelium Conference
  • Place of Presentation: Potsdam, Germany
  • Year and Date: 2014-08-04 – 2014-08-05
• [Presentation] Drk ファミリーとSTATa の関係性
  • Author(s): 岩出結実，荒川今日子，林俊成，佐々木儀充，荒木剛，石川恵，坂本祥太，Jeffrey Williams，川田健文
  • Organizer: 第３回日本細胞性粘菌学会
  • Place of Presentation: 京都大学理学研究科セミナーハウス、京都
• [Presentation] Dissection of the Dictyostelum STATc activation pathways.
  • Author(s): Araki, T., Hai, L.V., Sasaki, N., Na, J., Kawata, T., Eichinger, L. and Williams, J.G.
  • Organizer: International Dictyostelium Conference
  • Place of Presentation: Madrid, Spain
• [Presentation] Dissection of the Dictyostelium STATc activation pathways: Just Another Kinase for a non-metazoan STAT.
  • Author(s): Araki, T., Kawata, T. and Williams, J.G.
  • Organizer: 第2回日本細胞性粘菌学会年会
  • Place of Presentation: 東京大学駒場キャンパス 、東京