| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Social amoeba, Dictyostelium discoideum, harbors a number of tyrosine kinase-like kinases (TKLs). In this project, we analyzed three TKLs, DrkA, DrkC and RckA, that may phosphorylate tyrosine residue on a transcription factor STATa. We created a strain with a mutation of all three genes. The phosphorylation of the STATa was almost abolished in the mutant strain using a buffered artificial phosphorylation induction experiment. This indicates these TKLs play vital roles for STATa phosphorylation. However, STATa was phosphorylated in the mutant strain when it developed into a multicellular stage. Although we cannot explain this paradox at the moment, if the function of any particular TKL is inactivated then other TKL including unidentified novel one might replace it to show redundancy. To elucidate the redundancy mechanism would help to understand the new insights into evolutional acquisition process of protein tyrosine kinase.
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