Relationship between precursor folding and molecular evolution of a peptide hormone
Project/Area Number |
24510310
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Living organism molecular science
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Research Institution | Kinki University |
Principal Investigator |
HIDAKA Yuji 近畿大学, 理工学部, 教授 (70212165)
|
Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Hiroshi 関西学院大学, 理工学部, 教授 (10252719)
SHIMAMOTO Shigeru 近畿大学, 理工学部, 講師 (00610487)
|
Project Period (FY) |
2012-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | シャペロン / ジスルフィド結合 / フォールディング / ペプチドホルモン / 前駆体 / ウログアニリン / 分子内シャペロン / 分子進化 / 生物物理 / 生理活性ペプチド / 立体構造形成 / ジスルフィド / グアニリン |
Outline of Final Research Achievements |
To investigate how peptide hormones maintain/develop their biologically active conformation during molecular evolution, the folding of precursor proteins including prouroguanylin were estimated in vitro. Our results indicated that i)the stability of the biologically active conformation of the mature region is strongly related to the correct folding of their precursor proteins, ii)precursor protein possesses a region to accelerate molecular evolution, iii)precursor protein possesses a region to inhibit molecular evolution, iv)correct folding of prevursor protein requires mis-folded molecular species to establish the correct conformation of prouroguanylin
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Report
(5 results)
Research Products
(88 results)
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[Journal Article] Inhibition of the functional interplay between ER oxidoreduclin-1 a (Ero1a) and protein disulfide isomerase (PDI) by the endocrine disruptor2014
Author(s)
Okumura M, Kadokura H, Hashimoto S, Yutani K, Kanemura S, Hikima T, Hidaka Y, Ito L, Shiba K, Masui S, Imai D, Imaoka S, Yamaguchi H, Inaba K
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Journal Title
Journal of Biological Chemistry
Volume: 26
Issue: 39
Pages: 27004-27018
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Solution Structural Studies of Soluble Extracellular Domain of Amyloid Precursor Protein2013
Author(s)
Kanemura, S.(発表者), Okumura, M., Imai, D., Hikima, T., Niinobe, M., Hidaka, Y., and Yamaguchi, H(代表)
Organizer
4th International Symposium on Diffraction Structural Biology
Place of Presentation
Fukiage Hall (名古屋)
Related Report
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[Presentation] SOLUTION STRUCTURAL STUDIES OF SOLUBLE EXTRACELLULAR DOMAIN OF AMYLOID PRECURSOR PROTEIN2013
Author(s)
Kanemura, S.(発表者), Okumura, M., Imai, D., Yutani, K., Hikima, T., Niinobe, M., Hidaka, Y., and Yamaguchi, H(代表)
Organizer
The12th Meeting of the Asian Crystallographic Association
Place of Presentation
香港(中国)
Related Report
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