Studies on the cell function with a novel fluorescent probes.

• Project/Area Number: 24510316
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Kanagawa University
• Principal Investigator: KAWAZOE Yoshinori 神奈川大学, 付置研究所, 研究員 (20402927)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 分子イメージング / 蛍光化合物 / 分子プローブ / オルガネラ / 蛍光プローブ / ミトコンドリア / 微絨毛 / イメージング / 誘導展開 / 蛍光

Outline of Final Research Achievements

The aims of this study are changing the property of the fluorescent reagent I found through making derivatives and elucidation of cellular function with the compounds. I prepared several derivatives and succeeded to change their activated pH or fluorescent color. And I found one of them showed novel feature, staining the microvilli.

Research Products

• [Journal Article] New indole alkaloids from the sponge Plakortis sp. (2015)
  • Author(s): Hanif, N., Yamada, K., Kitamura, M., Kawazoe, Y., Voogd, N. J., Uemura, D.
  • Journal Title: Chem. Nat. Comp+ Volume: in press
• [Journal Article] A chemical probe that labels human pluripotent stem cells. (2014)
  • Author(s): Hirata, N., Nakagawa, M., Fujibayashi, Y., Yamauchi, K., Murata, A., Minami, I., Tomioka, M., Kondo, T., Kuo, T. F., Endo, H., Inoue, H., Sato, S., Ando, S., Kawazoe, Y., Aiba, K., Nagata, K., Kawase, E., Chang, Y. T., Suemori, H., Eto, K., Nakauchi, H., Yamanaka, S., Nakatsuji, N., Ueda, K., and Uesugi, M.
  • Journal Title: Cell Rep. Volume: 6 Pages: 1165-1174
  • NAID: 120005466668
  • Peer Reviewed
• [Journal Article] Design, synthesis, and evaluation, derivatives of the fat-accumulation inhibitor ternatin: toward ternatin molecular probes. (2014)
  • Author(s): Kawazoe, Y., Tanaka, Y., Omura, S., and Uemura, D.
  • Journal Title: Tetrahedron Lett. Volume: 55 Pages: 4445-4447
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] An inhibitor of the adipogenic differentiation of 3T3-L1 cells, yoshinone A, and its analogs, isolated from the marine cyanobacterium Leptolyngbya sp. (2014)
  • Author(s): Inuzuka, T., Yamamoto, K., Iwasaki, A., Ohno, O., Suenaga, K., Kawazoe, Y., and Uemura, D.
  • Journal Title: Tetrahedron Lett. Volume: 55 Pages: 6711-6714
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A chemical probe that labels human pluripotent stem cells (2014)
  • Author(s): Hirata N, Nakagawa M, Fujibayashi Y, Yamauchi K, Murata A, Minami I, Tomioka M, Kondo T, Kuo TF, Endo H, Inoue H, Sato S, Ando S, Kawazoe Y, Aiba K, Nagata K, Kawase E, Chang YT, Suemori H, Eto K, Nakauchi H, Yamanaka S, Nakatsuji N, Ueda K, Uesugi M
  • Journal Title: Cell reports Volume: 6 Issue: 6 Pages: 1165-74
  • DOI: 10.1016/j.celrep.2014.02.006
  • NAID: 120005466668
  • Peer Reviewed / Open Access
• [Journal Article] Small-molecule-induced clustering of heparan sulfate promotes cell adhesion (2013)
  • Author(s): Takemoto N, Suehara T, Frisco HL, Sato S, Sezaki T, Kusamori K, Kawazoe Y, Park SM, Yamazoe S, Mizuhata Y, Inoue R, Miller GJ, Hansen SU, Jayson GC, Gardiner JM, Kanaya T, Tokitoh N, Ueda K, Takakura Y, Kioka N, Nishikawa M, Uesugi M.
  • Journal Title: J. Am. Chem. Soc. Volume: 135 Issue: 30 Pages: 11032-11039
  • DOI: 10.1021/ja4018682
  • Peer Reviewed
• [Journal Article] 3. Jolkinolide F, a cytotoxic diterpenoid from Euphorbia jolkinii. (2013)
  • Author(s): Maru, N., Chikaraishi, N., Yokota, K., Kawazoe, Y., and Uemura, D.
  • Journal Title: Chem. Lett. Volume: 42 Issue: 7 Pages: 756-757
  • DOI: 10.1246/cl.130280
  • NAID: 10031183284
  • Peer Reviewed
• [Journal Article] エリブリン（ハラヴェン）：海洋天然物からの創薬展開 (2013)
  • Author(s): 川添嘉徳、上村大輔
  • Journal Title: 細胞工学 Volume: 32 Pages: 675-681
• [Journal Article] Guidelines for the useand interpretation of assays for monitering autophagy (2012)
  • Author(s): Klionsky, D.J. ほか
  • Journal Title: Autophagy Volume: 8 Pages: 445-544
  • Peer Reviewed
• [Presentation] 脂質代謝を制御する天然物yoshinone A (2015)
  • Author(s): 川添嘉徳、大野修、岩崎有紘、末永聖武、上村大輔
  • Organizer: 日本化学会 弟95春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 脂質代謝を調節する天然有機化合物群 (2014)
  • Author(s): 川添嘉徳、田中陽子、山本啓太、岩崎有紘、大野修、大村幸和、末永聖武、上村大輔
  • Organizer: 第56回天然有機化合物討論会
  • Place of Presentation: 高知
  • Year and Date: 2014-10-15 – 2014-10-17
• [Presentation] HalichromeAの合成研究 (2014)
  • Author(s): 鈴木佑太郎、穴澤雄太、阿部孝宏、川添嘉徳、渡部多恵子、山田薫、上村大輔
  • Organizer: 日本化学会第94回春季年会
  • Place of Presentation: 名古屋
• [Presentation] 脂肪細胞分化阻害を示す海洋性天然物 (2014)
  • Author(s): 山本啓太、丸範人、川添嘉徳、上村大輔
  • Organizer: 日本化学会第94回春季年会
  • Place of Presentation: 名古屋
• [Presentation] 脂肪蓄積阻害活性物質テルナチンのケミカルバイオロジー (2014)
  • Author(s): 田中陽子、片山知昭、川添嘉徳、上村大輔
  • Organizer: 日本化学会第94回春季年会
  • Place of Presentation: 名古屋
• [Presentation] タンパク質リン酸化酵素GSK-3betaの阻害活性を有する渦鞭毛藻由来天然物探索 (2014)
  • Author(s): 山本啓太、大島貴則、川添嘉徳、上村大輔
  • Organizer: 日本化学会第94回春季年会
  • Place of Presentation: 名古屋
• [Presentation] Chemotherapy for cancer stem cells. (2013)
  • Author(s): Yoshinori Kawazoe and Daisuke Uemura
  • Organizer: Anricancer Drugs 2013
  • Place of Presentation: Stockholm, Sweden
• [Presentation] Chemical Biology of a cyclic peptide exhibiting fat accumulation inhibition (2013)
  • Author(s): Yoshinori Kawazoe, Yoko Tanaka, and Daisuke Uemura
  • Organizer: International Symposium on Natural Products Chemistry and Chemical Biology 2013
  • Place of Presentation: Nagoya
• [Presentation] 脂肪蓄積阻害物質テルナチンのケミカルバイオロジー研究
  • Author(s): 田中陽子、片山知昭、川添嘉徳、上村大輔
  • Organizer: 新規素材探索研究会代12回セミナー
  • Place of Presentation: 横浜
• [Presentation] クロイソカイメン由来の生物活性物質探索
  • Author(s): 山本啓太、丸範人、川添嘉徳
  • Organizer: 新規素材探索研究会代12回セミナー
  • Place of Presentation: 横浜
• [Presentation] 脂肪蓄積阻害活性物質テルナチンのケミカルバイオロジー研究
  • Author(s): 田中陽子、増田駿、川添嘉徳、上村大輔
  • Organizer: 第2回CSJ化学フェスタ
  • Place of Presentation: 東京工業大学
• [Presentation] 脂肪蓄積阻害活性物質テルナチンのケミカルバイオロジー
  • Author(s): 田中陽子、片桐圭祐、斉藤直紀、川添嘉徳、上村大輔
  • Organizer: 日本化学会第93回春期年会
  • Place of Presentation: 立命館大学
• [Presentation] クロイソカイメン由来の微量生物活性物質
  • Author(s): 山本啓太、丸範人、川添嘉徳、上村大輔
  • Organizer: 日本化学会第93回春期年会
  • Place of Presentation: 立命館大学
• [Presentation] 渦鞭毛藻の大量培養とシンビオイミンの生理活性
  • Author(s): 横田圭祐、川添嘉徳、上村大輔
  • Organizer: 日本化学会第93回春期年会
  • Place of Presentation: 立命館大学
• [Presentation] 小分子化合物による細胞機能解析
  • Author(s): 川添嘉徳
  • Organizer: 平成24年度第一回舎密会
  • Place of Presentation: 神奈川大学
  • Invited
• [Patent(Industrial Property Rights)] 抗肥満剤 (2015)
  • Inventor(s): 上村大輔、川添嘉徳、小山智之、末永聖武、岩崎有紘
  • Industrial Property Rights Holder: 上村大輔、川添嘉徳、小山智之、末永聖武、岩崎有紘
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-002278
  • Filing Date: 2015-01-08
• [Patent(Industrial Property Rights)] N-スルフォニルインドール誘導体化合物およびその用途 (2015)
  • Inventor(s): 上村大輔、阿部孝宏、川添嘉徳、渡部多恵子、鈴木祐太郎、山田薫
  • Industrial Property Rights Holder: 上村大輔、阿部孝宏、川添嘉徳、渡部多恵子、鈴木祐太郎、山田薫
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-042828
  • Filing Date: 2015-03-04
• [Patent(Industrial Property Rights)] 抗肥満薬 (2014)
  • Inventor(s): 上村大輔、川添嘉徳、丸範人、山本啓太
  • Industrial Property Rights Holder: 上村大輔、川添嘉徳、丸範人、山本啓太
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-137892
  • Filing Date: 2014-07-03
• [Patent(Industrial Property Rights)] Ｎ－スルフォニルインドール誘導体化合物およびその用途 (2014)
  • Inventor(s): 上村大輔、阿部孝宏、川添嘉徳、渡部多恵子、鈴木佑太郎、山田薫
  • Industrial Property Rights Holder: 上村大輔、阿部孝宏、川添嘉徳、渡部多恵子、鈴木佑太郎、山田薫
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-042177
  • Filing Date: 2014-03-04
• [Patent(Industrial Property Rights)] Ｎ－置換スルフォニルインドール誘導体化合物の新規用途 (2014)
  • Inventor(s): 上村大輔、阿部孝宏、川添嘉徳、渡部多恵子、鈴木佑太郎、山田薫
  • Industrial Property Rights Holder: 上村大輔、阿部孝宏、川添嘉徳、渡部多恵子、鈴木佑太郎、山田薫
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-042178
  • Filing Date: 2014-03-04