Development of cell membrane penetrating peptides

• Project/Area Number: 24510317
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: HASEGAWA Makoto 長浜バイオ大学, バイオサイエンス学部, 教授 (10367899)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: バイオプローブ / 機能性ペプチド

Outline of Final Research Achievements

Artificial peptides designed for cell penetrating have been developed by using systematic directed evolution in vitro, a cDNA display method. A surface plasmon resonance-based biosensor and fluorescence correlation spectroscopy to examine binding of peptides to POPC with high affinity. This study describes a new strategy for the development of artificial functional peptides, which are promising materials in biochemical analyses and medical applications.

Research Products

• [Journal Article] A Novel Tamoxifen Derivative, Ridaifen-F, Is a Nonpeptidic Small-molecule Proteasome Inhibitor (2014)
  • Author(s): Makoto Hasegawa, Yukari Yasuda, Makoto Tanaka, Kenya Nakata, Eri Umeda, Yanwen Wang, Chihiro Watanabe, Shoko Uetake,Tatsuki Kunoh, Masafumi Shionyu, Ryuzo Sasaki, Isamu Shiina, Tamio Mizukami
  • Journal Title: European Journal of Medicinal Chemistry Volume: 71 Pages: 290-305
  • DOI: 10.1016/j.ejmech.2013.11.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Regulation of TFPI-2 (Tissue Factor Pathway Inhibitor 2) expression by Lysine-Specific Demethylase 1 and 2 (LSD1 and LSD2). (2014)
  • Author(s): 2.Mino, K., Nishimura, S., Ninomiya, S., Tujii, H., Matsumori, Y., Tsuchida, M., Hosoi, M., Koseki, K., Wada, S., Hasegawa, M., Sasaki, R., Murakami-Yamaguchi, Y., Narita, H., Suzuki, T., Miyata, N., Mizukami, T.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 78 Issue: 6 Pages: 1010-1017
  • DOI: 10.1080/09168451.2014.910104
  • Peer Reviewed
• [Journal Article] Metal mesh device sensor immobilized with a trimethoxysilane-containing glycopolymer for label-free detection of proteins and bacteria (2014)
  • Author(s): Hirokazu Seto, Seiji Kamba, Takashi Kondo, Makoto Hasegawa, Shigeki Nashima, Yoshinobu Ehara, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura
  • Journal Title: ACS Applied Materials & Interfaces Volume: 6 Issue: 15 Pages: 13234-13241
  • DOI: 10.1021/am503003v
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of a Selective Inhibitor of KDM2/7 Histone Lysine Demethylases and its Antiproliferative Activity. (2013)
  • Author(s): Suzuki T, Ozasa H, Itoh Y, Zhan P, Sawada H, Mino K, Walport L, Ohkubo R, Kawamura A, Yonezawa M, Tsukada Y, Tumber A, Nakagawa H, Hasegawa M, Sasaki R, Mizukami T, Schofield CJ, Miyata N
  • Journal Title: Journal of Medicinal Chemistry Volume: 56 Issue: 18 Pages: 7222-7231
  • DOI: 10.1021/jm400624b
  • Peer Reviewed / Open Access
• [Journal Article] Biotinyiation of silicon and nickel surfaces and detection of streptavidin as biasensor. (2013)
  • Author(s): Hirokazu Seto, Chie Yamashita, Seiji Karnba, Takashi Kondo, Makoto Hasegawa, Mitsuhiro Matsuno, Yuichii Ogawa, Yu Hoshino, Yoshiko Miura
  • Journal Title: Langmuir Volume: 29 Issue: 30 Pages: 9457-9463
  • DOI: 10.1021/la401068n
  • Peer Reviewed
• [Journal Article] Lysine-Specific Demethylase 1-Selective Inactivators Based on Protein-Targeted Drug Delivery Mechanism (2013)
  • Author(s): Ogasawara D, Itoh Y, Tsumoto H, Kakizawa T, Mino K, Fukuhara K, Nakagawa H, Hasegawa M, Sasaki R, Mizukami T, Miyata N, Suzuki T
  • Journal Title: Angewandte Chemie International Edition Volume: 52 Issue: 33 Pages: 8620-8624
  • DOI: 10.1002/anie.201303999
  • Peer Reviewed
• [Journal Article] Alarin but not its alternative-splicing form, GALP (Galanin- like peptide) has antimicrobial activity. (2013)
  • Author(s): A. Wada, Y. Kuboら
  • Journal Title: Biochemical and Biophysical Research Communication Volume: 434 Issue: 2 Pages: 223-227
  • DOI: 10.1016/j.bbrc.2013.03.045
  • Peer Reviewed
• [Presentation] リダイフェン-Fのプロテアソーム阻害作用向上のための新しい方法 (2015)
  • Author(s): 田中誠、椎名勇、佐々木隆造、水上民夫 、長谷川慎
  • Organizer: 第19回日本がん分子標的治療学会学術集会
  • Place of Presentation: 愛媛県
  • Year and Date: 2015-06-10 – 2015-06-12
• [Presentation] ヘリコバクター・ピロリの産生毒素VacAに対するペプチドアプタマーの探索と評価 (2015)
  • Author(s): 早川結実子，松野充宏，田中誠，武居修，北村幸一郎，長谷川慎
  • Organizer: 第62回日本生化学会近畿支部例会
  • Place of Presentation: 滋賀県
  • Year and Date: 2015-05-16
• [Presentation] 周期構造金属薄膜を用いた細胞分離と赤外線透過性による検出 (2015)
  • Author(s): 山本晃毅，森康介，白井恵美，井上有香，萬壽優，渡邉順子， 神波誠治，近藤孝志，長谷川慎
  • Organizer: 第62回日本生化学会近畿支部例会
  • Place of Presentation: 滋賀県
  • Year and Date: 2015-05-16
• [Presentation] 新規プロテアソーム阻害剤リダイフェン-Fの構造活性相関研究 (2015)
  • Author(s): 田中誠，杉山茂仁，早川結実子，水澤彰人, 渡邊千尋, 太田のぞみ, 椎名勇，佐々木隆造，水上民夫，長谷川慎
  • Organizer: 第62回日本生化学会近畿支部例会
  • Place of Presentation: 滋賀県
  • Year and Date: 2015-05-16